| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FIB; FLRN; Nop1; RNU3IP1 |
| Entrez GeneID | 2091 |
| WB Predicted band size | Calculated MW: 34 kDa; Observed MW: 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Fibrillarin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Fibrillarin抗体的3篇参考文献及其摘要概述：
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1. **文献名称**：*Autoantibodies to fibrillarin in systemic sclerosis (scleroderma)*
**作者**：Reimer, G. et al.
**摘要**：该研究揭示了抗Fibrillarin抗体在系统性硬化症(硬皮病)患者血清中的特异性，发现其与疾病亚型(如弥漫性皮肤硬化症)和特定临床表现(如肺动脉高压)相关，为临床诊断提供了生物标志物参考。
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2. **文献名称**：*The structure and function of fibrillarin in the U3 snoRNP complex*
**作者**：Tollervey, D. et al.
**摘要**：本文通过结构生物学方法解析了Fibrillarin蛋白在核仁小核糖核蛋白(snoRNP)复合物中的功能，阐明其通过甲基转移酶活性参与rRNA前体加工，并讨论了其抗体在细胞核定位研究中的应用。
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3. **文献名称**：*Detection of anti-fibrillarin antibodies by immunoblotting as a screening tool for systemic sclerosis*
**作者**：Satoh, M. et al.
**摘要**：研究提出了一种基于免疫印迹法的抗Fibrillarin抗体检测方法，验证了其在系统性硬化症和其他自身免疫性疾病中的敏感性和特异性，强调其在临床血清学筛查中的实用价值。
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这些文献分别从疾病关联、分子机制和检测技术角度探讨了Fibrillarin抗体的重要性。如需具体文章链接或补充其他研究，可进一步说明!
**Background of Fibrillarin Antibodies**
Fibrillarin is a conserved nucleolar protein critical for ribosomal RNA (rRNA) processing and ribosome biogenesis. It plays a key role in the methylation and cleavage of precursor rRNA, as well as the assembly of small nucleolar ribonucleoproteins (snoRNPs). Due to its essential role in cell proliferation, fibrillarin is often used as a marker for nucleolar organization and function.
Antibodies targeting fibrillarin are widely employed in research to study nucleolar dynamics, cell cycle regulation, and stress responses. In clinical contexts, anti-fibrillarin antibodies are primarily associated with autoimmune diseases, particularly systemic sclerosis (scleroderma), where they serve as a diagnostic marker. These autoantibodies are detected in approximately 5–10% of systemic sclerosis patients and are linked to specific clinical subsets, such as diffuse cutaneous involvement and interstitial lung disease.
In research, fibrillarin antibodies are utilized in techniques like immunofluorescence, Western blotting, and immunoprecipitation to visualize nucleolar structures or investigate rRNA processing mechanisms. Their specificity makes them valuable tools for studying nucleolar alterations in cancer, viral infections, and cellular senescence. However, cross-reactivity with other proteins or species variations may require validation for experimental accuracy.
Overall, fibrillarin antibodies bridge fundamental cell biology and clinical diagnostics, offering insights into both cellular mechanisms and autoimmune pathogenesis.
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