| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ACTR3; ARP3; Actin-related protein 3; Actin-like protein 3 |
| Entrez GeneID | 10096 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Arp3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Actin Related Protein 3(ARP3)抗体的代表性文献摘要(人工归纳，非真实文献，仅作示例)：
1. **文献名称**：*"Role of Arp3 in lamellipodia formation and cell migration"*
**作者**：Suraneni et al.
**摘要**：通过特异性ARP3抗体干扰实验，验证了Arp2/3复合体在哺乳动物细胞片状伪足形成中的必要性，并揭示了ARP3缺失导致细胞迁移能力下降的机制。
2. **文献名称**：*"Arp2/3 complex structure and actin filament branching revealed by antibody mapping"*
**作者**：Robinson et al.
**摘要**：利用多克隆ARP3抗体进行免疫共沉淀和结构分析，阐明了Arp2/3复合体的三维构象及其在调控肌动蛋白网络分支中的分子机制。
3. **文献名称**：*"Overexpression of ARP3 correlates with poor prognosis in glioma"*
**作者**：Wu et al.
**摘要**：通过免疫组化(IHC)和Western blot(WB)结合商业化ARP3抗体，发现胶质瘤组织中ARP3蛋白高表达与患者生存期缩短显著相关。
4. **文献名称**：*"Antibody-based inhibition of Arp3 disrupts metastatic processes in melanoma"*
**作者**：Rotty & Bear
**摘要**：开发了靶向ARP3的单克隆抗体，证明其可有效阻断黑色素瘤细胞的侵袭性伪足形成，为癌症治疗提供了潜在策略。
(注：以上文献为示例性内容，实际引用需检索真实数据库如PubMed)
Actin-Related Protein 3 (ARP3) is a crucial subunit of the Arp2/3 complex, a conserved multi-protein assembly essential for nucleating and branching actin filaments in eukaryotic cells. This complex regulates cytoskeletal dynamics, enabling processes like cell motility, membrane trafficking, and cytokinesis. ARP3 antibodies are immunological tools designed to detect and study the expression, localization, and function of ARP3 in various biological contexts. These antibodies, often raised in rabbits or mice, are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to investigate cytoskeletal reorganization in diseases like cancer, immune disorders, and neurodegenerative conditions.
Research utilizing ARP3 antibodies has highlighted its role in cancer metastasis, where aberrant Arp2/3 activity promotes invasive cell migration. They also aid in studying developmental defects linked to cytoskeletal dysregulation. Specificity is typically validated using knockout cell lines or siRNA-mediated depletion. Recent studies explore ARP3's interaction with nucleation-promoting factors (e.g., WASP, SCAR/WAVE) and its involvement in autophagy or pathogen infection mechanisms. As the Arp2/3 complex gains attention as a potential therapeutic target, ARP3 antibodies remain vital for dissecting its pathophysiological contributions across experimental models.
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