| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII |
| Entrez GeneID | 4846 |
| clone | 6E8 |
| WB Predicted band size | Calculated MW: 133 kDa; Observed MW: 133 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Protein of eNOS |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3条关于eNOS抗体的参考文献示例(文献信息为模拟内容，实际引用需核实)：
1. **文献名称**: *"Characterization of a monoclonal antibody to endothelial nitric oxide synthase"*
**作者**: Sessa WC et al.
**摘要**: 本研究开发并验证了一种特异性识别eNOS的单克隆抗体，通过免疫印迹和免疫组化证实其对人、大鼠和小鼠内皮细胞中eNOS的高效检测能力，为研究血管内皮功能提供了工具。
2. **文献名称**: *"Regulation of endothelial nitric oxide synthase expression by antibody targeting in vascular cells"*
**作者**: Shaul PW et al.
**摘要**: 利用eNOS抗体研究其在动脉粥样硬化模型中的表达变化，发现抗体介导的检测可定量eNOS蛋白水平与活性，揭示氧化应激对eNOS稳定性的调控机制。
3. **文献名称**: *"Phosphorylation-dependent modulation of eNOS activity detected by site-specific antibodies"*
**作者**: Fulton D et al.
**摘要**: 通过位点特异性eNOS抗体分析磷酸化修饰(如Ser1177)对酶活性的影响，证实抗体在动态监测eNOS激活状态中的应用价值，为心血管疾病研究提供分子依据。
4. **文献名称**: *"Antibody-based detection of eNOS dimerization in endothelial dysfunction"*
**作者**: Michel T et al.
**摘要**: 研究利用eNOS抗体结合非变性电泳技术，揭示eNOS二聚体/单体比例在糖尿病模型中的失衡机制，提示抗体检测可作为评估内皮功能障碍的生物标志物。
**提示**：实际文献需通过PubMed或Google Scholar检索关键词(如“eNOS antibody”、“eNOS detection”)获取，建议参考《Circulation Research》《Journal of Biological Chemistry》等期刊的高被引论文。
Endothelial nitric oxide synthase (eNOS) is a critical enzyme encoded by the *NOS3* gene, primarily expressed in vascular endothelial cells. It catalyzes the production of nitric oxide (NO), a key signaling molecule regulating vasodilation, blood flow, and vascular homeostasis. eNOS-derived NO also modulates platelet aggregation, inflammation, and angiogenesis, making it essential for cardiovascular health. Dysregulation of eNOS activity is linked to pathologies like hypertension, atherosclerosis, and diabetes.
eNOS antibodies are immunological tools designed to detect and quantify eNOS protein levels in research applications. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to study eNOS expression, localization, and post-translational modifications (e.g., phosphorylation at Ser1177 or Thr495) that influence enzyme activity. These antibodies are critical for investigating eNOS dysfunction in disease models, assessing endothelial cell responses to stimuli (shear stress, hypoxia, drugs), or validating gene-editing outcomes.
Available as monoclonal or polyclonal forms, eNOS antibodies vary in specificity across species (human, mouse, rat) and require validation for cross-reactivity. Researchers often use them alongside antibodies targeting phosphorylated eNOS to dissect activation pathways (e.g., Akt/PI3K or AMPK signaling). Reliable eNOS antibodies are indispensable for advancing studies on endothelial biology, drug development, and mechanisms underlying vascular diseases.
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