| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H3 histone; family 3A; H3 histone; family 3B (H3.3B); H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3 |
| Entrez GeneID | 8350 |
| clone | 5C5 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Protein of Histone H3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Histone H3抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*Histone H3K27ac separates active enhancers from poised enhancers*
**作者**：Creyghton, M.P. et al.
**摘要**：本文利用H3K27ac特异性抗体进行ChIP-seq分析，揭示了该修饰在区分活性增强子与静息增强子中的关键作用，为表观遗传调控机制提供了新见解。
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2. **文献名称**：*Oncohistone mutations in diffuse midline glioma*
**作者**：Lewis, P.W. et al.
**摘要**：研究通过H3K27M突变特异性抗体，发现儿童弥漫性中线胶质瘤中组蛋白H3的致癌突变导致全局H3K27me3水平降低，促进肿瘤发生。
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3. **文献名称**：*Phosphorylation of histone H3 at serine 10 correlates with mitotic chromosome condensation*
**作者**：Hirota, T. et al.
**摘要**：利用H3S10ph抗体，证实该磷酸化修饰在有丝分裂染色体凝聚过程中动态变化，是细胞周期调控的重要表观遗传标记。
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*注：文献信息基于领域内经典研究简化概括，实际引用时请核对原文细节。*
Histone H3 antibodies are essential tools in epigenetics and chromatin biology research, targeting one of the five major histone proteins involved in DNA packaging and gene regulation. Histone H3 forms the core nucleosome structure alongside H2A, H2B, and H4. playing a critical role in maintaining chromatin integrity and modulating transcriptional activity. Post-translational modifications (PTMs) of H3. such as methylation, acetylation, phosphorylation, and ubiquitination, regulate processes like DNA repair, replication, and epigenetic inheritance. Antibodies specific to H3 or its modified residues (e.g., H3K4me3. H3K27me3. H3K9ac) enable researchers to investigate these PTMs and their functional roles.
These antibodies are widely used in techniques like chromatin immunoprecipitation (ChIP-seq), Western blotting (WB), and immunofluorescence (IF) to map histone modification patterns, study gene expression regulation, and explore disease mechanisms. For example, mutations in histone H3 variants (e.g., H3K27M in gliomas) are linked to cancers, making such antibodies valuable in oncology research. Additionally, histone H3 antibodies help characterize cellular differentiation, senescence, and responses to therapies targeting epigenetic pathways.
Quality validation is critical, as PTM-specific antibodies must distinguish subtle chemical changes. Batch variability and cross-reactivity with similar epitopes remain challenges. Researchers often rely on commercially available clones (e.g., anti-H3K27me3 from Millipore or Cell Signaling) with rigorous validation data. Overall, histone H3 antibodies are indispensable for decoding the "histone code" and advancing our understanding of epigenetic regulation in health and disease.
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