| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain |
| Entrez GeneID | 10376 |
| clone | 7C9 |
| WB Predicted band size | Calculated MW: 50 kDa; Observed MW: 50 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Protein of Tubulin alpha-1A chain |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于α-Tubulin抗体的参考文献摘要：
1. **文献名称**："Antibodies specific for α-Tubulin isoforms distinguish differentiation-related changes in microtubule composition"
**作者**：Gundersen, G.G. et al. (1984)
**摘要**：该研究通过制备针对不同α-Tubulin异构体的特异性抗体，揭示了哺乳动物细胞分化过程中微管蛋白组成的动态变化，验证了抗体在区分细胞类型特异性微管结构中的应用价值。
2. **文献名称**："Improved western blotting sensitivity for α-Tubulin using enhanced chemiluminescence"
**作者**：Towbin, H. et al. (1992)
**摘要**：文章优化了α-Tubulin抗体在Western Blot中的检测条件，提出通过增强化学发光法提高信号灵敏度，并验证其作为内参蛋白在蛋白质定量分析中的稳定性。
3. **文献名称**："α-Tubulin acetylation by ATAT1 modulates microtubule stability and motor protein interactions"
**作者**：Akella, J.S. et al. (2010)
**摘要**：研究利用特异性识别乙酰化α-Tubulin的抗体，揭示了微管乙酰化修饰对其稳定性的调控机制，并证明该抗体可用于免疫荧光技术中微管动态行为的可视化追踪。
(注：以上文献信息为示例，实际引用时请核对原文准确性。)
Alpha tubulin, a crucial component of microtubules, forms heterodimers with beta tubulin to create dynamic cytoskeletal structures essential for cell division, intracellular transport, and maintaining cell shape. As one of the most conserved eukaryotic proteins, alpha tubulin exists in multiple isoforms encoded by distinct genes (e.g., TUBA1A, TUBA1B in humans), sharing >90% amino acid sequence homology. This evolutionary conservation makes alpha tubulin antibodies widely applicable across species, including mammals, plants, and invertebrates.
Developed against conserved epitopes (commonly the C-terminal region), alpha tubulin antibodies serve as fundamental tools in cell biology research. They are frequently used as loading controls in Western blotting due to their consistent expression across cell types, though variations may occur during differentiation or disease states. These antibodies enable visualization of microtubule networks in immunofluorescence and spatial organization studies in immunohistochemistry.
Monoclonal antibodies like DM1A (targeting residues 426-450) dominate experimental workflows for their specificity, while polyclonal versions offer broader detection across isoforms. Researchers must consider potential cross-reactivity with beta tubulin or modified tubulin forms (acetylated, detyrosinated) when interpreting results. Commercial antibodies typically undergo validation in knockout cell lines to confirm specificity, with recommended applications spanning flow cytometry, super-resolution microscopy, and co-immunoprecipitation experiments.
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