| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GP180; L CA; CD45 antigen |
| Entrez GeneID | 19264 |
| clone | 9D3 |
| WB Predicted band size | Calculated MW: 145 kDa; Observed MW: 145 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic Peptide of CD45 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于CD45抗体的3篇代表性文献(示例仅供参考，建议通过学术数据库获取原文)：
1. **文献名称**：*CD45: A critical regulator of signaling thresholds in immune cells*
**作者**：Trowbridge IS, Thomas ML
**摘要**：该综述总结了CD45作为跨膜酪氨酸磷酸酶在免疫细胞信号传导中的核心作用，重点探讨其通过调控Src家族激酶活性影响T细胞和B细胞受体信号通路的机制。
2. **文献名称**：*CD45 isoform expression in human leukemia*
**作者**：Beverley PC, Linch D, Delia D
**摘要**：研究通过单克隆抗体技术分析不同CD45剪接异构体(如CD45RA、CD45RO)在白血病细胞表面的表达差异，揭示其作为白血病分型标志物的潜在价值。
3. **文献名称**：*CD45-deficient mice reveal essential roles for CD45 in T cell activation and transplantation tolerance*
**作者**：Kishihara K, Penninger J
**摘要**：通过基因敲除小鼠模型证明CD45缺失导致T细胞活化障碍，并发现CD45在移植免疫耐受中的关键调控作用，为自身免疫疾病研究提供理论依据。
(注：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台检索)
CD45. also known as leukocyte common antigen (LCA), is a transmembrane protein tyrosine phosphatase expressed on nearly all hematopoietic cells except mature erythrocytes and platelets. It plays a critical role in regulating immune cell signaling by modulating the activity of Src family kinases (e.g., Lck, Fyn) involved in T-cell and B-cell receptor pathways. CD45 is essential for lymphocyte development, activation, and differentiation, acting as both a positive and negative regulator of immune responses depending on cellular context.
CD45 antibodies are widely used in research and diagnostics to identify and characterize leukocytes. Its pan-leukocyte expression makes it a key marker in flow cytometry and immunohistochemistry to distinguish hematopoietic cells from non-hematopoietic tissues. In clinical settings, CD45 antibodies aid in diagnosing hematologic malignancies, such as leukemia and lymphoma, by confirming the leukocyte origin of tumor cells. Additionally, CD45 isoforms (e.g., CD45RA, CD45RO) are employed to differentiate naive and memory T-cell subsets, providing insights into immune status and disease progression.
Research applications include studying immune dysregulation in autoimmune diseases, transplant rejection, and immunodeficiency disorders. CD45's conserved structure across species also facilitates translational studies. However, its diverse splicing variants and context-dependent functions necessitate careful antibody selection to target specific epitopes or isoforms. Overall, CD45 antibodies remain indispensable tools in immunology and hematopathology.
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