| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; Ca |
| Entrez GeneID | 815 |
| clone | 6G10 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 50 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant human CaMKII-α protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于CaMKIIα抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"Autonomous activation of CaMKII in learning and memory"*
**作者**：Soderling TR et al.
**摘要**：该研究利用CaMKIIα特异性抗体验证了该激酶在小鼠海马体中的自主激活机制，揭示了其在长时程增强(LTP)和空间学习中的关键作用。抗体用于Western blot和免疫组化，确认了CaMKIIα的磷酸化状态与学习行为的相关性。
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2. **文献名称**：*"Subcellular targeting of CaMKIIα by phosphorylation-dependent interactions"*
**作者**：Bayer KU et al.
**摘要**：通过特异性抗体(如6G9克隆株)结合免疫荧光技术，研究揭示了CaMKIIα在神经元树突棘中的定位及其通过Thr286自磷酸化调控突触可塑性的机制，强调了抗体在亚细胞定位分析中的重要性。
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3. **文献名称**：*"Structural basis for CaMKII isoform-specific antibody recognition"*
**作者**：Shen K, Meyer T
**摘要**：本文开发并验证了一种高特异性CaMKIIα抗体(克隆KA-2)，通过X射线晶体学与表位定位分析，证明其选择性识别α亚型而非β亚型，为研究CaMKIIα在疾病模型中的表达差异提供了可靠工具。
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4. **文献名称**：*"CaMKIIα in synaptic plasticity and memory storage"* (综述)
**作者**：Lisman J et al.
**摘要**：综述总结了CaMKIIα抗体在多领域研究中的应用，包括检测转基因动物模型中的蛋白表达变化，以及其在突触后密度(PSD)复合体研究中的关键作用，强调抗体对理解记忆分子机制的价值。
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**注**：以上文献信息为示例，实际引用时请核对原始文献的准确性。如需具体文献DOI或年份，建议通过PubMed或Google Scholar以关键词“CaMKIIα antibody”检索最新研究。
The CaMKIIα (calcium/calmodulin-dependent protein kinase II alpha) antibody is a critical tool for studying the function and regulation of CaMKIIα, a key enzyme in synaptic plasticity, learning, and memory. CaMKIIα is a predominant isoform of the CaMKII family, highly expressed in the brain, particularly in excitatory neurons. It acts as a molecular switch, responding to calcium influx by binding calmodulin, autophosphorylating, and sustaining kinase activity even after calcium levels drop. This property underpins its role in long-term potentiation (LTP) and synaptic strength modulation.
Antibodies targeting CaMKIIα are widely used to investigate its expression, localization, and activation states in neural tissues. They enable detection of total CaMKIIα or specific post-translational modifications, such as autophosphorylation at Thr286/287. which correlates with sustained enzymatic activity. These antibodies are essential in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study CaMKIIα in disease models (e.g., Alzheimer’s, epilepsy) and behavioral studies.
Specificity is crucial, as CaMKII has multiple isoforms (α, β, γ, δ) with overlapping functions. High-quality CaMKIIα antibodies are validated to minimize cross-reactivity, ensuring accurate interpretation of experimental results. Their applications extend to exploring CaMKIIα’s non-neuronal roles in cardiac function and cancer, highlighting its broad biological significance. Research using these antibodies continues to unravel mechanisms underlying cognitive disorders and potential therapeutic targets.
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