Mouse Monoclonal FEN1 Antibody

The Flap Endonuclease 1 (FEN1) antibody is a critical tool for studying the FEN1 protein, a multifunctional enzyme essential for DNA replication and repair. FEN1 plays a pivotal role in processing Okazaki fragments during DNA replication by cleaving 5' flap structures, ensuring lagging-strand synthesis fidelity. It also participates in base excision repair (BER), resolving DNA lesions caused by oxidative or alkylating agents. Dysregulation of FEN1 is linked to genomic instability, cancer, and neurodegenerative disorders, making it a focus of biomedical research. FEN1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and interactions (e.g., with PCNA or DNA polymerase δ). These antibodies help elucidate FEN1's role in cellular responses to DNA damage, chemotherapy resistance, and disease mechanisms. Both monoclonal and polyclonal variants are available, often validated using knockout cell lines or recombinant proteins to ensure specificity. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to avoid false signals. As FEN1 emerges as a potential biomarker or therapeutic target in cancers, reliable antibodies remain indispensable for advancing molecular diagnostics and drug development studies.