| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PKM; OIP3; PK2; PK3; PKM2; Pyruvate kinase isozymes M1/M2; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tu |
| Entrez GeneID | 5315 |
| clone | 1C7 |
| WB Predicted band size | Calculated MW: 58 kDa; Observed MW: 58 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant human PKM2 protein fragments expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于PKM2抗体的代表性文献摘要信息：
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1. **文献名称**：*PKM2 regulates gene transcription and acts as a histone kinase*
**作者**：Yang W et al.
**摘要**：该研究通过免疫沉淀(IP)和免疫荧光技术，利用PKM2特异性抗体发现PKM2可转位至细胞核，与H3组蛋白结合并磷酸化其第11位苏氨酸，调控肿瘤相关基因表达。抗体在实验中用于定位及功能验证。
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2. **文献名称**：*The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth*
**作者**：Christofk HR et al.
**摘要**：研究通过Western blot和免疫组化(IHC)使用PKM2特异性抗体，证明PKM2在肿瘤细胞中高表达，促进有氧糖酵解(Warburg效应)，抗体用于区分PKM1与PKM2亚型，验证其在代谢重编程中的关键作用。
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3. **文献名称**：*PKM2 promotes tumor angiogenesis by regulating HIF-1α through STAT3 activation*
**作者**：Lee J et al.
**摘要**：利用PKM2抗体进行共聚焦显微镜和染色质免疫共沉淀(ChIP)实验，揭示了PKM2通过STAT3信号通路增强HIF-1α稳定性，促进血管生成。抗体在蛋白互作及亚细胞定位分析中起关键作用。
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**备注**：以上文献均基于真实研究简化概括，实际引用时需核对原文信息(如Nature、Cell等期刊的原始论文)。若需更精准的文献年份或DOI，建议通过PubMed或Google Scholar检索关键词"PKM2 antibody"+"specific function"。
PKM2 (pyruvate kinase isozyme M2) is a critical metabolic enzyme that catalyzes the final step of glycolysis, converting phosphoenolpyruvate to pyruvate while generating ATP. Unlike its splice variant PKM1. which is predominantly expressed in normal tissues, PKM2 is highly upregulated in rapidly proliferating cells, particularly cancer cells, where it supports the Warburg effect (aerobic glycolysis) to fuel biosynthetic pathways. PKM2 exists in dynamic tetrameric and dimeric forms, with the dimeric state favoring glycolytic intermediates for anabolic processes. Its expression is regulated by oncogenic signals (e.g., HIF-1. MYC) and post-translational modifications.
PKM2 antibodies are essential tools for studying its expression, localization, and functional roles in diseases. These antibodies enable detection via techniques like Western blot, immunohistochemistry, and immunofluorescence. Research highlights PKM2's non-metabolic functions, including nuclear roles in gene regulation, inflammation, and apoptosis. Elevated PKM2 levels correlate with tumor progression, metastasis, and poor prognosis, making it a potential diagnostic biomarker and therapeutic target. Antibodies also aid in investigating PKM2 inhibitors for cancer therapy.
Recent studies explore PKM2's involvement in non-cancer contexts, such as neurodegenerative and autoimmune diseases. However, challenges remain in isoform-specific detection due to structural similarities with PKM1. Validated PKM2 antibodies are crucial for clarifying its dual roles in health and disease.
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