| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAPK3 |
| Entrez GeneID | 5595 |
| clone | 1G5 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant protein expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于ERK1抗体的经典文献示例(内容为模拟概括，实际文献请以数据库检索为准)：
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1. **文献名称**: "Purification and characterization of extracellular signal-regulated kinase 1/2-specific antibodies"
**作者**: Crews, C.M., et al.
**摘要**: 该研究报道了针对ERK1/2蛋白C末端结构域的多克隆抗体的制备与验证。抗体成功应用于Western blot和免疫沉淀实验，特异性识别ERK1(44 kDa)和ERK2(42 kDa)，为研究MAPK信号通路提供了关键工具。
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2. **文献名称**: "Differential subcellular localization of ERK1 and ERK2 in response to mitogenic stimuli"
**作者**: Robinson, M.J., et al.
**摘要**: 通过使用ERK1特异性抗体，研究发现ERK1与ERK2在生长因子刺激后呈现不同的核转位动态，提示两者在信号转导中可能具有非冗余功能，抗体在免疫荧光实验中验证了其亚细胞定位特异性。
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3. **文献名称**: "Phosphorylation and activation of ERK1 by MEK1 in vitro"
**作者**: Rubinfeld, H., et al.
**摘要**: 研究利用ERK1抗体检测体外激酶反应中ERK1的磷酸化状态(Thr202/Tyr204)，证实MEK1对ERK1的激活机制，抗体在非变性条件下仍能识别活性与非活性形式的ERK1.支持了功能研究的需求。
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*注：以上文献信息为示例性质，实际引用时建议通过PubMed或Google Scholar检索真实文献(关键词：ERK1 antibody, MAPK3. Western blot validation)。*
ERK1 (Extracellular Signal-Regulated Kinase 1), also known as MAPK3. is a serine/threonine kinase within the mitogen-activated protein kinase (MAPK) family. It plays a central role in the RAS-RAF-MEK-ERK signaling cascade, which regulates critical cellular processes such as proliferation, differentiation, survival, and apoptosis. ERK1 is activated through dual phosphorylation at Thr202 and Tyr204 residues by upstream MAPK/ERK kinases (MEK1/2) in response to extracellular stimuli like growth factors, cytokines, or stress signals. Once activated, ERK1 translocates to the nucleus to phosphorylate transcription factors (e.g., c-Fos, c-Jun) or interacts with cytoplasmic targets to modulate cellular responses.
ERK1 antibodies are essential tools for studying this pathway in biomedical research. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect ERK1 expression, phosphorylation status (using phospho-specific antibodies), and subcellular localization. Commercial ERK1 antibodies are often developed against conserved epitopes, such as the C-terminal region, to ensure specificity across species (human, mouse, rat). Some antibodies distinguish between ERK1 (44 kDa) and its closely related isoform ERK2 (42 kDa). Validation includes knockout controls or siRNA-mediated silencing to confirm target specificity. These antibodies are crucial in cancer research, neuroscience, and studies of diseases linked to dysregulated MAPK signaling, such as neurodegenerative disorders and inflammatory conditions.
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