首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠S100-A6蛋白(S100A6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的S100A6含量。S100A6是一个重要靶点。它在多种生理和病理过程中有作用，如细胞的增殖、分化等。相关研究聚焦于其与疾病发生发展的关联机制，试图明确其在信号通路中的角色，为开发针对该靶点的治疗方法提供理论基础。试剂盒检测范围为2.4 ng/mL-150 ng/mL，可广泛应用于神经生物学、肿瘤机制研究及炎症反应等领域的科研实验，例如在动物模型中分析S100A6在不同疾病状态下的动态表达水平，或探究其在细胞应激反应中的调节作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：S100A6 ELISA Kit; Protein S100-A6 ELISA Kit; Calcyclin ELISA Kit; Lung 10 kDa protein ELISA Kit; S100 calcium-binding protein A6 ELISA Kit
缩写：S100A6
Uniprot No.：P30801
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：2.4 ng/mL-150 ng/mL
灵敏度：0.6 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat S100A6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:2 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:4 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:8 | Average % | 112 | |||||
| Range % | 109-115 | ||||||
回收率：
| The recovery of rat S100A6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 83 | 80-86 | |||||
| EDTA plasma (n=4) | 95 | 91-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected1502.7002.7652.7332.570751.9851.9351.9601.79737.51.3411.3201.3311.16818.750.8700.8680.8690.7069.40.5260.5410.5340.3714.70.3950.3820.3890.2262.40.2470.2350.2410.07800.1620.1640.163 | |||||||
货期：3-5 working days
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