| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP1LC3B |
| Entrez GeneID | 81631 |
| clone | 3F8 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant protein expressed in E.coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于LC3B抗体的3篇参考文献概览：
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1. **文献名称**：Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
**作者**：Klionsky, D.J. et al.
**摘要**：该指南系统总结了自噬研究的关键方法，包括LC3B抗体在免疫印迹和免疫荧光中的应用。强调需结合溶酶体抑制剂(如氯喹)验证自噬通量，并指出不同抗体可能因识别LC3B-I/II异构体差异而影响结果解读。
2. **文献名称**：Comparing the performance of different commercial antibodies for detecting LC3B in autophagy studies
**作者**：Shibutani, S.T. & Sato, T.
**摘要**：研究比较了多种市售LC3B抗体的特异性，发现部分抗体存在交叉反应(如与MAP1B蛋白)，推荐使用CST公司的#3868抗体以提高免疫组化及Western blot的可靠性。
3. **文献名称**：LC3B antibody validation in tissue samples: Pitfalls and solutions
**作者**：Nakamura, S. et al.
**摘要**：探讨LC3B抗体在石蜡包埋组织中的检测挑战，指出抗体克隆(如5F10 vs. D11)对结果影响显著，建议结合基因敲除样本验证抗体特异性，避免假阳性。
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**备注**：LC3B抗体研究需关注实验体系(细胞/组织)、抗体克隆及验证方法。Klionsky指南为方法学基石，后两篇则聚焦抗体选择与验证的实际问题。
LC3B (Microtubule-associated protein 1A/1B-light chain 3B) is a critical biomarker for studying autophagy, a cellular self-degradation process that maintains homeostasis. As a mammalian homolog of yeast Atg8. LC3B exists in two forms: cytosolic LC3-I and lipidated LC3-II, which integrates into autophagosome membranes during autophagy. LC3-II formation is a hallmark of autophagosome maturation, making it a key target for monitoring autophagic activity.
LC3B antibodies are widely used to detect and quantify autophagosomes via techniques like Western blotting, immunofluorescence, and immunohistochemistry. These antibodies distinguish between LC3-I (18 kDa) and LC3-II (16 kDa), with increased LC3-II levels or punctate staining patterns indicating enhanced autophagy. However, interpreting results requires caution, as LC3B accumulation may reflect either autophagy induction or impaired lysosomal degradation.
Researchers often combine LC3B detection with other autophagy markers (e.g., p62/SQSTM1) to assess autophagic flux—the dynamic process of autophagosome formation and degradation. LC3B antibodies are essential tools in studying autophagy-related diseases, including cancer, neurodegenerative disorders, and infections. Their specificity and reliability depend on experimental conditions, such as cell fixation methods and inhibitor use (e.g., bafilomycin A1 to block lysosomal activity).
In summary, LC3B antibodies provide critical insights into autophagy regulation and dysfunction, bridging molecular mechanisms to pathophysiological outcomes.
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