| WB | 1/500-1/1000 | Human,Mouse,Rat,Chicken |
| IF | 咨询技术 | Human,Mouse,Rat,Chicken |
| IHC | 1/50-1/100 | Human,Mouse,Rat,Chicken |
| ICC | 1/50-1/200 | Human,Mouse,Rat,Chicken |
| FCM | 咨询技术 | Human,Mouse,Rat,Chicken |
| Elisa | 1/10000 | Human,Mouse,Rat,Chicken |
| Aliases | AI849689; c Jun N terminal kinase 1; C-JUN kinase 1; c-Jun N-terminal kinase 1; EC 2.7.11.24; JAK 1A; JAK1A; JNK 1; JNK 46; JNK; JNK-46; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; MAPK8; Mitogen activated protein kinase 8; Mitogen-activated protein kinase 8; MK08_HUMAN; p54 gamma; PRKM 8; PRKM8; Protein kinase JNK1; Protein kinase; mitogen-activated; 8; SAPK 1; SAPK gamma; SAPK1; Stress activated protein kinase JNK1; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1; Tyrosine protein kinase JAK1 . |
| Entrez GeneID | 5599 |
| WB Predicted band size | Calculated MW: 48 kDa; Observed MW: 46,54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Chicken |
| Immunogen | The antiserum was produced against synthesized peptide derived from human JNK1/2/3 around the phosphorylation site of Thr183 and Tyr185. AA range:151-200 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-JNK (Thr183/Tyr185)抗体的3篇参考文献示例(文献信息为模拟示例，供参考)：
1. **"Role of JNK activation in oxidative stress-induced apoptosis"**
*Authors: Davis R.J., et al.*
摘要：研究通过Phospho-JNK (Thr183/Tyr185)抗体检测JNK磷酸化水平，揭示氧化应激下JNK信号激活促进细胞凋亡的机制。
2. **"Mechanical stress activates JNK signaling in cardiac myocytes"**
*Authors: Wang Y., et al.*
摘要：利用Phospho-JNK特异性抗体，证实机械应力通过Thr183/Tyr185位点磷酸化激活JNK，进而调控心肌细胞肥大反应。
3. **"Phosphorylation dynamics of JNK in neuronal injury models"**
*Authors: Harper S.J., et al.*
摘要：通过Western blot和免疫荧光技术，结合Phospho-JNK抗体，分析神经元损伤模型中JNK活化时空分布及其与神经退行性病变的关联。
4. **"Inhibition of JNK phosphorylation attenuates inflammatory responses in macrophages"**
*Authors: Kim E.K., et al.*
摘要：研究使用Phospho-JNK (Thr183/Tyr185)抗体验证抑制剂对JNK通路的阻断效果，证明JNK磷酸化抑制可减少炎症因子释放。
(注：以上文献为示例性内容，实际引用需根据具体论文补充。)建议通过PubMed或Google Scholar以关键词“Phospho-JNK Thr183 Tyr185 antibody”或“JNK activation”检索近期研究。
Phospho-JNK (Thr183/Tyr185) antibodies are essential tools for studying the activation status of c-Jun N-terminal kinase (JNK), a member of the mitogen-activated protein kinase (MAPK) family. JNKs, including isoforms JNK1. JNK2. and JNK3. are activated in response to cellular stressors (e.g., UV radiation, cytokines, oxidative stress) and play critical roles in apoptosis, inflammation, and differentiation. Their activation requires dual phosphorylation at conserved Thr183 and Tyr185 residues within the activation loop. Antibodies targeting these phosphorylated sites specifically detect the active, phosphorylated form of JNK, enabling researchers to investigate JNK signaling dynamics in various contexts.
These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess JNK activation in cell lysates, tissue samples, or experimental models. Their applications span cancer research (e.g., studying pro-apoptotic signaling), neurobiology (e.g., neuronal stress responses), and immunology (e.g., inflammatory pathways). Validation often includes testing knockout controls or phosphatase-treated samples to confirm specificity. As dysregulated JNK activity is implicated in diseases like Alzheimer’s, cancer, and autoimmune disorders, Phospho-JNK antibodies are vital for both mechanistic studies and therapeutic target validation.
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