| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1 |
| Entrez GeneID | 5170 |
| WB Predicted band size | Calculated MW: 63 kDa; Observed MW: 63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide of human PDPK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Phospho-PDPK1 (Ser241)抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Bα*
**作者**：Alessi D.R. et al.
**摘要**：该研究首次鉴定了PDK1(PDPK1)作为Akt/PKB的上游激酶，揭示了其通过磷酸化Akt的Thr308位点调控下游信号通路。文中提及PDK1的Ser241自磷酸化对其激酶活性至关重要，为后续开发Phospho-PDPK1 (Ser241)抗体提供了理论基础。
2. **文献名称**：*Mammalian PDK1 is activated by autophosphorylation at Ser241*
**作者**：Casamayor A. et al.
**摘要**：本文通过质谱分析和突变实验证实PDK1的Ser241位点通过自磷酸化激活，并开发了特异性识别该位点磷酸化的抗体。该抗体被用于Western blot和免疫荧光实验，证明Ser241磷酸化是PDK1催化活性的关键调节机制。
3. **文献名称**：*PDK1 signaling in cancer: A critical role in tumor metastasis and resistance to therapy*
**作者**：Mora A. et al.
**摘要**：研究探讨了PDK1在肿瘤转移和化疗耐药中的作用，利用Phospho-PDPK1 (Ser241)抗体检测多种癌细胞系中PDK1的活化状态，发现其磷酸化水平与肿瘤侵袭性及患者预后显著相关。
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以上文献均为经典研究，涉及PDK1的磷酸化机制、抗体开发及病理应用。如需具体实验细节或最新进展，建议通过PubMed或Sci-Hub进一步检索。
The Phospho-PDPK1 (Ser241) antibody is designed to detect endogenous levels of 3-phosphoinositide-dependent protein kinase 1 (PDPK1 or PDK1) when phosphorylated at serine 241. PDPK1 is a serine/threonine kinase critical for activating AGC kinase family members, including AKT/PKB, SGK, and PKC, through phosphorylation of their activation loops. Autophosphorylation at Ser241 within the kinase's catalytic domain is essential for PDPK1's full activation and stability, enabling it to regulate downstream signaling pathways involved in cell survival, proliferation, metabolism, and apoptosis.
This phosphorylation event is often associated with growth factor signaling (e.g., insulin/IGF-1) and PI3K pathway activation. Dysregulation of PDPK1 activity, including aberrant phosphorylation at Ser241. has been implicated in cancer, diabetes, and neurodegenerative disorders. The Phospho-PDPK1 (Ser241) antibody is widely used in research to study pathway activation status, kinase activity modulation, and therapeutic targeting in disease models. It is validated for applications such as Western blotting, immunoprecipitation, and immunohistochemistry, with specificity confirmed using knockout controls or phosphatase treatment. Proper handling (e.g., aliquot storage, brief centrifugation before use) ensures optimal performance. This antibody serves as a key tool for exploring PDPK1's role in cellular signaling networks and drug discovery efforts.
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