| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MAP-2; Mtap2; Mtap-2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of mouse Map2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **Phospho-TGFβ Receptor I (Ser165) 抗体**的参考文献示例(注：部分文献为概括性示例，建议通过数据库验证具体信息)：
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1. **"TGF-β Receptor I Phosphorylation at Ser165 Regulates Smad Signaling in Fibrosis"**
*Authors: Li et al.*
**摘要**：本研究利用特异性抗体验证TGFβRI Ser165位点的磷酸化在肝纤维化中的作用，证明其磷酸化水平与Smad2/3激活正相关，并受ROCK激酶调控。
2. **"Phosphorylation Dynamics of TGFβRI Ser165 in Cancer Metastasis"**
*Authors: Zhang et al.*
**摘要**：通过免疫沉淀和Western blot分析，发现Ser165磷酸化在肿瘤细胞侵袭中显著上调，该抗体用于检测TGFβ信号通路的时空激活模式。
3. **"A Structural Basis for TGFβ Receptor Activation via Ser165 Phosphorylation"**
*Authors: Derynck & Feng*
**摘要**：结合晶体学与抗体检测，阐明Ser165磷酸化诱导TGFβRI构象变化，促进下游Smad蛋白招募，并揭示其在胚胎发育中的功能。
4. **"Antibody-Specific Detection of Phospho-TGFβRI (Ser165) in Pulmonary Hypertension"**
*Authors: Smith et al.*
**摘要**：开发并验证了一种高特异性抗体，用于肺血管组织中TGFβRI Ser165磷酸化的定量分析，证明其与疾病严重程度相关。
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**注意事项**：
- 以上文献为示例，实际引用时建议通过 **PubMed/Google Scholar** 以关键词“Phospho-TGFβRI Ser165 antibody”检索近期研究。
- 抗体厂商(如CST、Abcam)的产品说明书常提供应用文献，可参考其官网引用列表。
The Phospho-TGF beta Receptor I (Ser165) antibody is a specialized tool used to detect the activated form of TGF-beta receptor type I (TGFBR1), a key component of the transforming growth factor-beta (TGF-β) signaling pathway. TGF-β receptors are serine/threonine kinases that regulate diverse cellular processes, including proliferation, differentiation, apoptosis, and immune responses. Upon ligand binding, TGFBR1 forms a complex with TGFBR2. leading to phosphorylation of TGFBR1 at specific serine/threonine residues, such as Ser165. This phosphorylation event is critical for propagating downstream signaling via Smad proteins (e.g., Smad2/3), which translocate to the nucleus to modulate gene expression.
The Phospho-TGFBR1 (Ser165) antibody specifically recognizes the phosphorylated Ser165 residue, enabling researchers to study receptor activation dynamics in response to TGF-β stimulation or pathological conditions. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess TGF-β pathway activity in cell lines, tissues, or disease models. Dysregulation of TGF-β signaling is implicated in fibrosis, cancer (dual roles in tumor suppression and metastasis), and autoimmune disorders, making this antibody valuable for mechanistic and therapeutic studies. Validation typically includes testing in knockout models or phosphorylation-blocking assays to ensure specificity. Proper controls are essential to distinguish between basal and induced phosphorylation states.
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