| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1 |
| Entrez GeneID | 5604 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MEK1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-MEK1 (Ser298)抗体的3篇假设参考文献，结合领域内经典研究框架整理：
1. **文献名称**：*"PAK Phosphorylation of MEK1 Regulates Raf/MEK/ERK Signaling and Cell Proliferation"*
**作者**：Shaw, A.E. et al.
**摘要**：研究证明PAK激酶通过磷酸化MEK1的Ser298位点，增强其与RAF的结合能力，从而促进ERK信号通路的激活和细胞增殖。该文献使用Phospho-MEK1 (Ser298)抗体验证了该位点的磷酸化依赖性相互作用。
2. **文献名称**：*"A Critical Role for MEK1 Ser298 Phosphorylation in Fibroblast Migration and Focal Adhesion Dynamics"*
**作者**：Slack-Davis, J.K. et al.
**摘要**：通过Phospho-MEK1 (Ser298)特异性抗体，发现该位点的磷酸化受FAK信号调控，并影响MEK1在细胞前沿的定位，进而调节成纤维细胞的迁移和黏着斑重塑。
3. **文献名称**：*"Distinct Phosphorylation States of MEK1 Mediate Signaling Specificity in the MAPK Pathway"*
**作者**：Fukushima, T. et al.
**摘要**：文章对比了MEK1不同磷酸化位点(如Ser218/222与Ser298)的功能差异，利用位点特异性抗体证明Ser298磷酸化不直接激活MEK1酶活，但参与其与下游支架蛋白的结合，影响信号通路的时空特异性。
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**备注**：以上文献为示例性质，实际研究中建议通过PubMed或抗体厂商(如CST #9128)提供的参考文献获取具体信息。真实文献可能需结合具体实验背景筛选。
The Phospho-MEK1 (Ser298) antibody detects MEK1 (MAPK/ERK kinase 1) when phosphorylated at serine 298. a regulatory site critical for its function in the RAS/RAF/MEK/ERK signaling cascade. MEK1. a dual-specificity kinase, acts as a central node in transmitting extracellular signals (e.g., growth factors, cytokines) to downstream effectors like ERK1/2. regulating cell proliferation, differentiation, and survival. Phosphorylation at Ser298. located in the kinase domain’s activation loop, is associated with MEK1 activation and its interaction with scaffolding proteins or upstream regulators like RAF kinases. This modification may enhance MEK1’s catalytic activity or stabilize its conformation for efficient ERK phosphorylation.
The antibody is widely used in cancer research, as hyperactivation of MEK1-ERK signaling is linked to tumorigenesis and drug resistance. It helps assess MEK1 activation status in cell lines, tissues, or preclinical models treated with pathway inhibitors (e.g., RAF or MEK inhibitors). Detection methods include Western blot, immunofluorescence, or immunohistochemistry. Researchers also use it to study feedback mechanisms, cross-talk with other pathways, or MEK1’s role in diseases beyond cancer, such as developmental disorders. Specificity validation (e.g., using phosphorylation-blocking mutants) is essential due to potential cross-reactivity with homologous sites in related kinases. Understanding Ser298 phosphorylation dynamics provides insights into MEK1 regulation and therapeutic targeting of aberrant signaling pathways.
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