| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | STAT1; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84 |
| Entrez GeneID | 6772 |
| WB Predicted band size | Calculated MW: 87 kDa; Observed MW: 87 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Phospho-STAT1 (S727) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Phospho-STAT1 (Ser727)抗体相关的文献示例(内容为模拟概括，建议核实原文)：
1. **文献名称**: "Role of serine phosphorylation in STAT1 transcriptional activity"
**作者**: Stark GR et al.
**摘要**: 研究STAT1在Ser727位点的磷酸化对其转录活性的调控，通过该抗体证实干扰素刺激后Ser727磷酸化与STAT1二聚体核转位的关系，揭示其与基因启动子结合的增强效应。
2. **文献名称**: "Distinct roles of STAT1 serine 727 phosphorylation in pro-inflammatory responses"
**作者**: Decker T, Kovacs B
**摘要**: 利用Phospho-STAT1 (Ser727)抗体在巨噬细胞模型中证明，该位点磷酸化通过调控线粒体代谢影响炎症因子表达，与Tyr701磷酸化形成功能互补。
3. **文献名称**: "STAT1 phosphorylation status determines apoptosis versus proliferation in cancer cells"
**作者**: Ramana CV et al.
**摘要**: 通过Western blot和免疫荧光使用该抗体，发现Ser727磷酸化的STAT1在DNA损伤条件下促进促凋亡基因表达，而Tyr701磷酸化单独不足以激活此通路。
(注：以上为基于领域知识的模拟摘要，实际文献需通过PubMed/Google Scholar以关键词“Phospho-STAT1 Ser727 antibody”检索，并核对抗体货号及具体实验条件。)
Phospho-STAT1 (Ser727) antibody is a key tool for studying the activation and regulation of Signal Transducer and Activator of Transcription 1 (STAT1), a transcription factor critical in mediating cellular responses to cytokines, growth factors, and interferons (IFNs). STAT1 is activated through phosphorylation at two primary sites: tyrosine 701 (Tyr701) and serine 727 (Ser727). Phosphorylation at Tyr701. mediated by Janus kinases (JAKs) or receptor tyrosine kinases, induces STAT1 dimerization, nuclear translocation, and DNA binding. In contrast, Ser727 phosphorylation occurs in the transactivation domain and is mediated by kinases such as mTOR, p38 MAPK, or CAMKII, enhancing transcriptional activity by promoting interactions with coactivators like p300/CBP. This post-translational modification also links STAT1 to non-canonical roles, including mitochondrial function and apoptosis regulation.
Phospho-STAT1 (Ser727)-specific antibodies enable researchers to detect and quantify STAT1 activation at this site using techniques like Western blotting, immunofluorescence, or flow cytometry. These antibodies are widely used to study immune responses, viral infections, and cancer, where dysregulated STAT1 signaling contributes to pathogenesis. For example, they help assess STAT1 activation in IFN-treated cells, autoimmune disorders, or tumors with constitutively active signaling pathways. Proper experimental controls (e.g., unphosphorylated STAT1 detection and kinase inhibition) are essential to confirm specificity. Understanding Ser727 phosphorylation provides insights into the nuanced regulation of STAT1-dependent gene expression and its broader cellular roles.
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