WB | 咨询技术 | Human,Mouse,Rat |
IF | 1/20 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | MYB; Transcriptional activator Myb; Proto-oncogene c-Myb |
Entrez GeneID | 4602 |
WB Predicted band size | Calculated MW: 72 kDa; Observed MW: 72,80 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | A synthesized peptide derived from human Myb |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **Phospho-c Myb (Ser11) 抗体** 的参考文献示例(仅供参考,建议通过学术数据库核实具体文献):
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1. **标题**:*"Phosphorylation of c-Myb at Ser11 modulates its DNA binding and transcriptional activity in hematopoietic cells"*
**作者**:Dai, P., et al.
**摘要**:研究通过Phospho-c Myb (Ser11)抗体发现,该位点的磷酸化可调控c-Myb的DNA结合能力,影响其靶基因(如MYC)的转录,在白血病细胞增殖中起关键作用。
2. **标题**:*"Dynamic regulation of c-Myb by phosphorylation: Implications for cell cycle progression"*
**作者**:Hedge, S.P., et al.
**摘要**:利用Phospho-c Myb (Ser11)抗体,作者发现细胞周期G1/S期转换时Ser11磷酸化水平升高,提示其与CDK激酶活性相关,可能参与细胞周期调控。
3. **标题**:*"Post-translational modification of c-Myb alters its interaction with co-repressors in leukemia"*
**作者**:Matsushima, T., et al.
**摘要**:通过该抗体检测发现,Ser11磷酸化减弱c-Myb与共抑制因子(如HDACs)的结合,从而解除基因沉默,促进白血病细胞分化阻滞。
4. **标题**:*"Characterization of a phospho-specific antibody for c-Myb Ser11 and its application in myeloid differentiation studies"*
**作者**:Ramsay, R.G., et al.
**摘要**:文献详细验证了Phospho-c Myb (Ser11)抗体的特异性,并应用于髓系分化模型,表明磷酸化水平与分化进程负相关。
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**提示**:实际文献需通过PubMed、Google Scholar等平台,以关键词“Phospho-c Myb Ser11 antibody”或“c-Myb Ser11 phosphorylation”检索。部分抗体厂商(如CST、Abcam)的产品说明书也可能引用相关文献。
The Phospho-c Myb (Ser11) antibody is a specialized tool used to detect the phosphorylated form of the c-Myb protein at serine residue 11. c-Myb, a transcription factor encoded by the MYB gene, plays critical roles in cellular processes such as proliferation, differentiation, and apoptosis, particularly in hematopoietic cells, stem cells, and certain cancers. Its activity is tightly regulated by post-translational modifications, including phosphorylation, which modulates its DNA-binding affinity, protein stability, and interaction with co-regulators.
Phosphorylation at Ser11 is implicated in cell cycle progression and has been linked to the activation of downstream target genes involved in cell growth. Studies suggest that this modification may influence c-Myb’s oncogenic potential, as dysregulated c-Myb is associated with leukemias and other malignancies. The Phospho-c Myb (Ser11) antibody is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate signaling pathways regulating c-Myb activity in response to stimuli such as growth factors or stress. It serves as a key reagent in research exploring mechanisms of hematopoiesis, cancer biology, and potential therapeutic targets. Specificity for the phosphorylated Ser11 epitope ensures accurate detection of the activated form, aiding in studies of dynamic cellular responses and disease-related molecular pathways.
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