| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
| Entrez GeneID | 5925 |
| WB Predicted band size | Calculated MW: 106 kDa; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr356 of human Rb |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与Phospho-Rb (Thr356)抗体相关的文献示例(内容基于公开研究概括，非真实文献，仅供参考)：
1. **文献名称**：*"Cyclin-dependent kinase regulation of Rb phosphorylation in cell cycle progression"*
**作者**：Lin et al.
**摘要**：研究CDK4/6介导的Rb蛋白Thr356位点磷酸化在G1/S期转换中的作用，通过Phospho-Rb (Thr356)抗体验证其磷酸化水平与细胞增殖的关系。
2. **文献名称**：*"Phosphorylation-specific antibodies reveal dynamic Rb signaling in tumorigenesis"*
**作者**：Dephoure et al.
**摘要**：开发并验证了Phospho-Rb (Thr356)抗体的特异性，证明其在乳腺癌组织中检测异常磷酸化Rb的应用，关联临床预后。
3. **文献名称**：*"Targeting Rb phosphorylation in cancer therapy: Insights from kinase assays"*
**作者**：Chen & Weinberg
**摘要**：通过体外激酶实验和免疫印迹(使用Phospho-Rb Thr356抗体)，阐明CDK抑制剂对Rb磷酸化及肿瘤细胞周期阻滞的影响。
4. **文献名称**：*"Validation of phospho-specific Rb antibodies for functional proteomics"*
**作者**：Cell Signaling Technology团队
**摘要**：技术类文献，系统验证Phospho-Rb (Thr356)抗体在流式细胞术、免疫荧光中的特异性，强调其在信号通路分析中的可靠性。
**提示**：实际文献需通过PubMed或抗体供应商官网(如CST、Abcam)的引用列表查询。
The Phospho-Rb (Thr356) antibody is a specialized tool used to detect the retinoblastoma protein (Rb) when phosphorylated at threonine 356. a post-translational modification critical for regulating cell cycle progression. Rb is a tumor suppressor protein that controls the G1-to-S phase transition by binding and inhibiting E2F transcription factors. Phosphorylation of Rb, mediated by cyclin-dependent kinases (CDKs) such as CDK4/6. disrupts this interaction, releasing E2F to activate genes required for DNA replication. Thr356 phosphorylation occurs during the mid-G1 phase and is a key marker of cell cycle commitment.
This antibody is widely used in cancer research to study Rb inactivation mechanisms, as hyperphosphorylation of Rb (due to CDK dysregulation) is a hallmark of many cancers. It helps assess cell proliferation status, drug responses (e.g., CDK4/6 inhibitors like palbociclib), and Rb signaling dynamics in diseases. Validated in techniques like Western blotting, immunofluorescence, and immunohistochemistry, the antibody’s specificity for the phosphorylated Thr356 epitope ensures accurate detection of active Rb inactivation. Its applications extend to understanding developmental biology, apoptosis, and therapeutic resistance, making it essential for studies linking cell cycle control to oncogenesis or targeted therapies.
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