关键信息
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基因名
rpsM
- 应用
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别名
rpsM; b3298; JW3260; 30S ribosomal protein S13; Small ribosomal subunit protein uS13
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种属
Escherichia coli
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表达系统
E. coli
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标签
N- GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7S9
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表达区间
2-118aa
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分子量
40 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RpsM, a ribosomal protein S13, plays a crucial role in the assembly and function of the ribosome, which is essential for protein synthesis in all living organisms. Research on RpsM has gained significance due to its involvement in ribosome biogenesis and its potential implications in understanding various cellular processes, including growth and response to stress. Mutations in rpsM can lead to ribosomal defects, impacting translational fidelity and efficiency, which may contribute to diseases, including cancers. Moreover, RpsM and its analogs have been investigated for their interactions with antibiotics, providing insights into antibiotic resistance mechanisms. The characterization of RpsM as a recombinant protein facilitates the study of its structural properties and functional interactions within the ribosomal complex. Through the expression and purification of RpsM, researchers can delve deeper into its biochemical properties, explore its role in ribosomal assembly, and assess its potential as a target for novel antimicrobial strategies. Understanding RpsM's function and interactions is not only vital for deciphering the intricacies of ribosomal machinery but also has broader implications in biotechnology and medicine, paving the way for advancements in treating ribosome-related disorders and enhancing antibiotic efficacy.
