Rabbit Monoclonal Phospho-HistoneH3(Ser10/Thr11) Antibody

Phospho-Histone H3 (Ser10/Thr11) antibodies are essential tools for studying cell cycle progression, particularly during mitosis. Histone H3. a core component of nucleosomes, undergoes post-translational modifications that regulate chromatin structure and gene expression. Phosphorylation at serine 10 (Ser10) and threonine 11 (Thr11) residues is tightly linked to chromatin condensation during mitosis. This modification peaks in the G2/M phase, marking cells undergoing chromosomal segregation. The phosphorylation event is mediated by Aurora B kinase, a key regulator of the mitotic checkpoint and cytokinesis. These antibodies enable specific detection of mitotic cells in techniques like immunofluorescence, flow cytometry, and Western blot, making them valuable in cancer research, where proliferative activity correlates with tumor aggressiveness. They also help investigate chromosomal instability in diseases or drug-treated models. The transient nature of this phosphorylation—rapidly removed upon mitotic exit—underscores its role as a dynamic marker of mitotic progression. Researchers utilize these antibodies to assess cell cycle perturbations, screen anti-mitotic therapies, or study developmental processes involving rapid cell division. Proper controls, including non-phosphorylated histone H3 detection, are critical to distinguish mitotic-specific signals from background noise.