| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PTPS |
| Entrez GeneID | 5805 |
| WB Predicted band size | Calculated MW: 16 kDa; Observed MW: 16 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser19 of human PTS/PTPS |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-PTS (Ser19)抗体的参考文献示例(内容为虚构，仅供参考)：
1. **文献名称**: *"Phosphorylation of 6-pyruvoyltetrahydropterin synthase at Ser19 regulates tetrahydrobiopterin biosynthesis"*
**作者**: Smith, J. et al.
**摘要**: 研究揭示了PTS蛋白在Ser19位点的磷酸化通过调控其酶活性影响四氢生物蝶呤(BH4)的生物合成，利用Phospho-PTS (Ser19)抗体证实了其在细胞应激条件下的磷酸化动态变化。
2. **文献名称**: *"A novel phosphorylation site in PTS modulates endothelial nitric oxide synthase coupling"*
**作者**: Zhang, L. et al.
**摘要**: 通过Phospho-PTS (Ser19)抗体的免疫印迹分析，发现内皮细胞中PTS的Ser19磷酸化通过调节BH4水平影响一氧化氮合酶功能，与血管舒张功能障碍相关。
3. **文献名称**: *"Kinase-dependent regulation of PTS activity in phenylalanine metabolism"*
**作者**: Tanaka, R. et al.
**摘要**: 研究利用Phospho-PTS (Ser19)抗体证明AMPK激酶介导的Ser19磷酸化可增强PTS酶活性，从而在苯丙氨酸代谢异常模型中维持BH4稳态。
4. **文献名称**: *"Development and application of a phospho-specific antibody for 6-pyruvoyltetrahydropterin synthase"*
**作者**: Johnson, M. et al.
**摘要**: 首次报道了Phospho-PTS (Ser19)抗体的开发与验证，并通过该抗体在脑组织样本中检测到Ser19磷酸化水平的病理特异性变化，提示其与神经退行性疾病的潜在关联。
(注：以上文献为示例，实际引用需以真实文献为准。)
The Phospho-PTS (Ser19) antibody is a specific tool used to detect phosphorylation of the PTS (phosphotyrosine substrate) protein at serine residue 19. PTS, also known as STYX (serine/threonine/tyrosine-interacting protein), is a pseudophosphatase involved in regulating cellular signaling pathways, including MAPK (mitogen-activated protein kinase) cascades. Unlike active phosphatases, PTS lacks catalytic activity due to critical amino acid substitutions, but it binds phosphorylated targets, modulating their activity or localization. Phosphorylation at Ser19 is thought to influence PTS interactions with signaling components, impacting processes like cell cycle regulation, stress responses, and apoptosis.
This antibody enables researchers to study the activation status and functional role of PTS in various contexts, such as cancer, neuronal development, or metabolic disorders. It is commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess phosphorylation-dependent protein interactions or pathway activation. Validation often includes testing in knockout cell lines or phosphatase-treated samples to confirm specificity.
Understanding PTS phosphorylation dynamics provides insights into how pseudophosphatases fine-tune signaling networks without enzymatic activity. The Phospho-PTS (Ser19) antibody thus serves as a critical reagent for exploring non-catalytic regulatory mechanisms in cell biology and disease pathogenesis.
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