WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | PTPN1; PTP1B; Tyrosine-protein phosphatase non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1B |
Entrez GeneID | 5770 |
WB Predicted band size | Calculated MW: 50 kDa; Observed MW: 50 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser378 of human PTP1B |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与Phospho-PTP1B (Ser378)抗体相关的文献摘要,供参考:
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1. **文献名称**: "Insulin-induced phosphorylation of PTP1B at Ser378 mediates its interaction with 14-3-3β"
**作者**: Tao J. et al.
**摘要**: 研究揭示了胰岛素信号激活后,PTP1B在Ser378位点的磷酸化促进其与分子伴侣14-3-3β的结合,从而抑制PTP1B的磷酸酶活性,增强胰岛素受体信号通路。
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2. **文献名称**: "Phosphorylation of PTP1B at Ser378 regulates cellular oxidative stress and cancer cell invasion"
**作者**: Liu Y. et al.
**摘要**: 发现Ser378磷酸化通过调控PTP1B的亚细胞定位(内质网膜结合能力),影响ROS(活性氧)水平及肿瘤细胞侵袭,为靶向PTP1B的癌症治疗提供新机制。
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3. **文献名称**: "Serine phosphorylation of PTP1B modulates lipid metabolism in obesity"
**作者**: Bandyopadhyay D. et al.
**摘要**: 利用Phospho-PTP1B (Ser378)抗体证实,高脂饮食诱导的肥胖模型中,Ser378磷酸化水平升高与肝脏胰岛素抵抗和脂质沉积密切相关。
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**备注**:若需具体文献来源(期刊、年份等),建议通过PubMed或Google Scholar以关键词“PTP1B Ser378 phosphorylation”检索最新研究。部分抗体厂商(如CST、Abcam)的技术文档也可能引用相关文献。
The Phospho-PTP1B (Ser378) antibody is designed to detect protein tyrosine phosphatase 1B (PTP1B) when phosphorylated at serine residue 378. a post-translational modification critical for regulating its enzymatic activity. PTP1B, encoded by the *PTPN1* gene, is a key regulator of metabolic signaling pathways, including insulin and leptin receptor signaling. It dephosphorylates receptor tyrosine kinases (e.g., insulin receptor, EGFR) and downstream effectors, thereby attenuating signaling cascades. Phosphorylation at Ser378. mediated by kinases such as AKT or ERK, induces a conformational change that inhibits PTP1B activity, enhancing downstream signaling. This modification is implicated in metabolic disorders (e.g., diabetes, obesity) and cancer progression.
The Phospho-PTP1B (Ser378) antibody enables researchers to study the activation status of PTP1B in cellular contexts, particularly under conditions of insulin resistance, oxidative stress, or oncogenic signaling. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess phosphorylation-dependent PTP1B regulation in tissues or cultured cells. Validated for specificity, this antibody helps elucidate mechanisms linking PTP1B modulation to disease pathways, offering insights for therapeutic targeting. Its application spans diabetes research, cancer biology, and studies on endoplasmic reticulum stress, where PTP1B’s role in protein folding and degradation is increasingly recognized.
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