| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a |
| Entrez GeneID | 8350 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于TriMethyl-Histone H3 (Lys27)抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*Role of histone H3 lysine 27 methylation in Polycomb-group silencing*
**作者**：Cao, R. et al.
**摘要**：该研究揭示了H3K27的三甲基化(H3K27me3)是Polycomb抑制复合体(PRC2)介导基因沉默的关键表观遗传标记，并证实了特异性抗体在染色质免疫沉淀(ChIP)中的应用，为研究发育和癌症中的基因调控提供了工具。
2. **文献名称**：*Polycomb complexes repress developmental regulators in murine embryonic stem cells*
**作者**：Boyer, L.A. et al.
**摘要**：文章通过H3K27me3抗体的ChIP-seq分析，证明Polycomb复合体在胚胎干细胞中通过H3K27三甲基化抑制发育相关基因，维持干细胞多能性，为表观遗传调控机制提供了实验依据。
3. **文献名称**：*SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex*
**作者**：Pasini, D. et al.
**摘要**：研究利用H3K27me3抗体验证了SUZ12在PRC2复合体中的必要性，表明其缺失导致H3K27三甲基化水平下降及靶基因异常激活，强调了该抗体在解析PRC2功能中的关键作用。
如需获取全文，建议通过PubMed或Sci-Hub等平台检索文献标题及作者。
TriMethyl-Histone H3 (Lys27) [H3K27me3] antibodies are essential tools for studying post-translational modifications associated with epigenetic regulation. Histone H3 lysine 27 trimethylation is a conserved marker of transcriptionally repressive chromatin, primarily catalyzed by Polycomb repressive complex 2 (PRC2), with EZH2 or EZH1 as the catalytic subunit. This modification plays a critical role in gene silencing, X-chromosome inactivation, and maintenance of stem cell pluripotency. Aberrant H3K27me3 levels are linked to developmental disorders and cancers, such as gliomas, where H3K27M mutations drive oncogenesis, and certain solid tumors with PRC2 dysregulation.
H3K27me3-specific antibodies are widely used in chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting to map repressive domains, assess PRC2 activity, or investigate crosstalk with activating marks like H3K4me3. They are critical for studying cellular differentiation, tumor progression, and epigenetic therapies targeting PRC2 (e.g., EZH2 inhibitors). Validation in knockout models or enzymatic demethylation controls is recommended due to potential cross-reactivity with other methylated residues. These antibodies also aid in diagnosing H3K27-altered cancers, highlighting their dual research and clinical utility.
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