| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EIF2; EIF2B; PPP1R67; EIF2beta; eIF-2-beta |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human EIF2S2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DiMethyl-Histone H3 (Lys36) 抗体的3-4篇文献示例(部分为虚构或简化内容，仅供参考)：
1. **"Selective recognition of histone H3K36 dimethylation by the MMR complex in DNA repair"**
*作者：Brown, A. et al.*
摘要：研究利用特异性H3K36me2抗体，通过免疫沉淀和质谱分析，揭示了H3K36me2在DNA损伤修复中招募错配修复蛋白MMR的机制。
2. **"A high-specificity antibody for detecting H3K36me2 in chromatin profiling"**
*作者：Johnson, R. et al.*
摘要：报道了一种新型H3K36me2抗体的开发与验证，通过肽阵列和ChIP-seq证明其特异性，并应用于胚胎干细胞中组蛋白修饰的动态分析。
3. **"H3K36 dimethylation suppresses cryptic transcription in yeast"**
*作者：Lee, S. & Smith, T.*
摘要：使用H3K36me2抗体进行染色质分析，发现其通过抑制异常转录起始维持基因组稳定性，揭示了表观遗传调控的保守机制。
4. **"Cross-species comparison of H3K36me2 distribution using a pan-specific antibody"**
*作者：Zhang, Y. et al.*
摘要：验证了一种跨物种H3K36me2抗体的广谱适用性，比较了哺乳动物、果蝇等模式生物中该修饰的分布差异及功能关联。
(注：以上文献为示例性质，实际文献需通过PubMed/Google Scholar等平台以关键词“H3K36me2 antibody”或“Histone H3 Lys36 dimethylation”检索。)
The DiMethyl-Histone H3 (Lys36) antibody is a key tool for studying histone H3 lysine 36 dimethylation (H3K36me2), a post-translational modification involved in epigenetic regulation. Histone H3K36 methylation plays critical roles in transcription elongation, mRNA splicing, DNA repair, and chromatin structure maintenance. Specifically, H3K36me2 is enriched across gene bodies and correlates with active transcription, serving as a marker for transcribed regions. It also helps suppress cryptic transcription initiation and guides chromatin-modifying enzymes, such as histone deacetylases, to maintain proper gene expression patterns.
This modification is catalyzed by methyltransferases like the NSD family (NSD1. NSD2. NSD3) and SETD1A, while demethylases such as KDM2A/B regulate its removal. Dysregulation of H3K36me2 has been linked to developmental disorders, cancers, and neurological diseases. For instance, mutations in NSD2 or altered H3K36me2 levels are associated with tumorigenesis and poor prognosis in multiple cancers.
The DiMethyl-Histone H3 (Lys36) antibody is widely used in chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to map H3K36me2 distribution, assess its dynamics during cellular processes, or investigate its role in disease. Specificity validation via knockout/knockdown controls is essential, as cross-reactivity with similar histone marks (e.g., H3K36me3) can occur. Researchers rely on this antibody to explore epigenetic mechanisms underlying gene regulation and disease pathology.
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