| WB | 咨询技术 | Mouse,Rat |
| IF | 咨询技术 | Mouse,Rat |
| IHC | 1/50-1/100 | Mouse,Rat |
| ICC | 1/50-1/200 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 1/10000 | Mouse,Rat |
| Aliases | CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6 |
| Entrez GeneID | 58918 |
| WB Predicted band size | Calculated MW: 50,17 kDa; Observed MW: 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from rat Caspase 9. AA range:323-372 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3-4条关于Cleaved-Caspase 9抗体的示例参考文献(内容为虚构示例，仅供格式参考)：
1. **文献名称**：*"Activation of Caspase-9 in Apoptosis Induced by Chemotherapeutic Agents"*
**作者**：Thompson, C.B. et al.
**摘要**：研究化疗药物诱导的细胞凋亡机制，通过Cleaved-Caspase 9抗体(Western blot)证实Caspase-9的激活是线粒体凋亡途径的核心事件。
2. **文献名称**：*"Role of Cleaved Caspase-9 in Neuronal Death Following Traumatic Brain Injury"*
**作者**：Liu, H. et al.
**摘要**：在大鼠脑损伤模型中，使用Cleaved-Caspase 9抗体(免疫组化)检测到神经元中活性Caspase-9的显著表达，表明其参与创伤后细胞凋亡。
3. **文献名称**：*"Selective Activation of Caspase-9 in Cancer Cell Lines by Targeted Nanoparticles"*
**作者**：Wang, X. et al.
**摘要**：开发靶向纳米颗粒诱导肿瘤细胞凋亡，通过Cleaved-Caspase 9抗体验证特异性激活Caspase-9.并减少对正常细胞的毒性。
4. **文献名称**：*"Caspase-9 Cleavage as a Biomarker for Apoptosis in Breast Cancer Tissues"*
**作者**：Rodriguez, M. et al.
**摘要**：分析乳腺癌组织样本，利用Cleaved-Caspase 9抗体(免疫印迹)量化活性Caspase-9水平，发现其与患者预后显著相关。
(注：若需真实文献，建议通过PubMed/Google Scholar检索关键词“Cleaved Caspase-9 antibody”+研究领域。)
Cleaved-Caspase 9 antibodies are essential tools for detecting the activated form of Caspase-9. a key protease in the intrinsic apoptotic pathway. Caspase-9 exists as an inactive zymogen (procaspase-9) under normal conditions. Upon apoptotic stimuli, such as DNA damage or cellular stress, cytochrome c released from mitochondria binds to Apaf-1. forming the apoptosome complex. This triggers procaspase-9 autocleavage at specific aspartic acid residues (e.g., Asp315 in humans), generating active cleaved Caspase-9 (p35/p37 fragments), which subsequently activates downstream effector caspases like Caspase-3 to execute apoptosis.
Cleaved-Caspase 9 antibodies are designed to recognize epitopes near the cleavage site, ensuring specificity for the activated form over the full-length precursor. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry to study apoptosis mechanisms in cancer, neurodegenerative diseases, and developmental biology. Their application helps assess the activation status of the intrinsic apoptotic pathway, evaluate drug efficacy in preclinical models, or diagnose pathological conditions linked to dysregulated apoptosis. Commercial antibodies are typically raised in rabbits or mice against synthetic peptides corresponding to the cleavage region, with validation in relevant cell/tissue models. Proper controls (e.g., apoptosis-induced vs. untreated samples) are critical to confirm antibody specificity.
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