| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Endo G like 1; ENDOGL1; ENDOGL2; Endonuclease G like 1; Endonuclease G like 2; ENGL; ENGLA; ENGLB; Exog; Nuclease EXOG;;EXOG |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human EXOG |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ENDOGL1/ENGL抗体的示例性参考文献(注：文献为虚拟示例，实际引用需检索数据库)：
1. **"Development of a monoclonal antibody targeting human ENDOGL1 for DNA repair studies"**
*作者：Smith J, et al. (2020)*
**摘要**：报道了一种新型抗ENDOGL1单克隆抗体的开发，验证了其在免疫印迹和免疫荧光中的应用，并证实ENDOGL1在DNA损伤修复中的核酸酶活性。
2. **"ENGL1 in mitochondrial genome maintenance: Insights from antibody-based localization"**
*作者：Lee S, et al. (2018)*
**摘要**：通过ENGL抗体进行亚细胞定位分析，发现ENGL1定位于线粒体，并参与生殖细胞中线粒体DNA的复制与修复。
3. **"Functional characterization of EndoG-like 1 in apoptosis using RNAi and antibody-mediated inhibition"**
*作者：Zhang Y, et al. (2016)*
**摘要**：结合特异性抗体阻断和基因沉默技术，揭示了ENDOGL1在细胞凋亡中切割核DNA片段化的非经典通路作用。
4. **"Antibody profiling of ENDOGL1 expression in human cancers"**
*作者：Brown K, et al. (2021)*
**摘要**：利用抗ENDOGL1抗体分析其在多种肿瘤组织中的表达水平，发现其表达缺失可能与基因组不稳定性相关。
**建议**：实际文献可通过PubMed或Google Scholar以关键词“ENDOGL1 antibody”或“ENGL1 antibody”检索，并筛选涉及抗体开发、验证或功能应用的论文。
ENDOGL1 (Endonuclease G-like 1), also termed ENGL, is a protein encoded by the ENDOGL1 gene located on human chromosome 11. It belongs to the GIV-YIG nuclease superfamily and shares structural homology with Endonuclease G (EndoG), a mitochondrial enzyme involved in apoptosis and DNA repair. The ENDOGL1 protein contains a conserved N-terminal MIT (microtubule-interacting and trafficking) domain and a C-terminal GIV-YIG catalytic domain, suggesting roles in nucleic acid processing or interaction with microtubule-associated proteins.
ENDOGL1 antibodies are immunological tools developed to detect and study this protein’s expression, localization, and function. Studies indicate ENDOGL1 may participate in DNA damage repair pathways, though its precise biological mechanisms remain under investigation. Some research links it to mitochondrial processes or stress responses, with potential implications in cancer, neurodegeneration, or aging.
ENGL antibodies are commonly used in techniques like Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF) to analyze protein levels in tissues/cell lines. Commercial antibodies are typically raised against recombinant ENDOGL1 fragments or synthetic peptides. Validation includes verifying specificity via knockout controls or siRNA knockdown. Despite growing interest, ENDOGL1’s functional relevance and disease associations require further exploration, driving demand for reliable antibodies to support mechanistic studies.
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