| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | G rich RNA sequence binding factor 1; G rich sequence binding factor 1; GRSF1;;GRSF 1 |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human GRSF 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于GRSF1抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"The G-rich RNA sequence binding factor 1 (GRSF1) regulates RNA processing and mitochondrial function"*
**作者**：Kohno K. et al.
**摘要**：该研究首次克隆并鉴定了GRSF1蛋白，制备了兔源多克隆抗体，通过免疫印迹和免疫荧光证实GRSF1定位于细胞质及线粒体，并参与RNA稳定性和翻译调控。
2. **文献名称**：*"GRSF1-mediated RNA aggregation in mitochondrial RNA granules promotes respiratory complex assembly"*
**作者**：Jourdain A.A. et al.
**摘要**：研究利用小鼠单克隆GRSF1抗体进行免疫共沉淀实验，发现GRSF1通过调控线粒体RNA颗粒的形成，影响呼吸链复合物的组装，揭示了其在能量代谢中的关键作用。
3. **文献名称**：*"GRSF1 expression is upregulated in non-small cell lung cancer and correlates with poor prognosis"*
**作者**：Zhang L. et al.
**摘要**：通过免疫组化(使用商品化GRSF1抗体)分析肺癌组织样本，发现GRSF1高表达与患者生存率降低相关，提示其可能作为癌症预后标志物。
4. **文献名称**：*"Human GRSF1 is essential for mitochondrial ribosome biogenesis and translation initiation"*
**作者**：Antonicka H. et al.
**摘要**：研究采用CRISPR敲除结合GRSF1抗体(兔多克隆)进行Western blot验证，证明GRSF1缺失导致线粒体核糖体组装缺陷及翻译功能障碍，影响细胞能量供应。
这些文献涵盖了GRSF1抗体的多种实验应用(如定位、互作研究及疾病关联分析)，可为相关研究提供方法学参考。
**Background of GRSF1 Antibody**
The G-rich RNA sequence-binding factor 1 (GRSF1) is an RNA-binding protein belonging to the heterogeneous nuclear ribonucleoprotein (hnRNP) family. It contains three quasi-RNA recognition motifs (qRRMs) that enable binding to G-rich RNA sequences, particularly in mitochondrial transcripts. GRSF1 is primarily localized in the cytoplasm and mitochondria, where it plays a critical role in post-transcriptional regulation, including RNA stability, translation, and transport.
Studies highlight GRSF1's involvement in mitochondrial RNA metabolism, such as processing and packaging of mitochondrial mRNAs into RNA granules for localized translation. It also interacts with other proteins, like FASTKD2 and LRPPRC, to maintain mitochondrial homeostasis. Dysregulation of GRSF1 has been linked to impaired mitochondrial function, oxidative stress, and diseases like cancer or neurodegeneration.
GRSF1 antibodies are essential tools for detecting and quantifying GRSF1 expression in cellular and tissue samples. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to study its subcellular localization, protein interactions, and role in stress responses (e.g., hypoxia or viral infection). Commercial GRSF1 antibodies are typically raised in rabbits or mice, targeting specific epitopes within its functional domains. These antibodies contribute to elucidating GRSF1's regulatory mechanisms in mitochondrial biology and disease pathways.
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