| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Katanin p60 ATPase containing subunit A1; Katanin p60 subunit A1; KATNA1; p60 katanin;;p60 Katanin |
| WB Predicted band size | 56 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human p60 Katanin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于p60katanin抗体的3篇参考文献及其摘要概括：
1. **"Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome via a WD40-containing subunit"**
- **作者**: McNally, F.J., & Vale, R.D.
- **摘要**: 本研究首次克隆并鉴定了p60katanin作为微管切割酶的核心催化亚基，开发了特异性抗体用于免疫定位实验，发现p60katanin在细胞分裂过程中富集于中心体，调控微管网络重组。
2. **"Characterization of the p60 subunit of katanin and its association with microtubule dynamics"**
- **作者**: Hartman, J.J., et al.
- **摘要**: 通过p60katanin抗体分析，作者揭示了p60与p80亚基的相互作用机制，证明其在体外和细胞内通过切割微管调控纺锤体形成，抗体被用于免疫印迹和免疫荧光验证蛋白表达及定位。
3. **"Microtubule-severing activity of katanin regulates dendritic morphogenesis in neurons"**
- **作者**: Sharp, D.J., & Ross, J.L.
- **摘要**: 研究利用p60katanin抗体探究其在神经元树突发育中的作用，发现抗体阻断实验可抑制微管切割，导致树突分支异常，表明p60katanin通过调控微管动力学影响神经形态发生。
4. **"Structural basis of microtubule severing by the AAA+ enzyme katanin"**
- **作者**: Roll-Mecak, A., & McNally, F.J.
- **摘要**: 结合p60katanin抗体的功能抑制实验，本文通过冷冻电镜解析了p60的ATP酶结构域与微管结合的分子机制，阐明其如何通过构象变化实现微管切割。
这些文献均涉及p60katanin抗体的应用，涵盖功能验证、亚细胞定位及分子机制研究。
The p60katanin antibody is a crucial tool in studying the katanin protein complex, which plays a vital role in microtubule dynamics. Katanin, named after the Japanese sword "katana," is a heterodimer composed of a p60 catalytic subunit (KATNA1) and a p80 regulatory subunit (KATNB1). The p60 subunit possesses ATPase activity and severs microtubules by hydrolyzing ATP, enabling cellular processes like mitosis, cell migration, and neuronal development. This severing activity is essential for reorganizing microtubule networks during cell cycle progression and maintaining cytoskeletal plasticity.
Antibodies targeting p60katanin are widely used to investigate its expression, localization, and function in diverse biological contexts. They enable researchers to visualize p60katanin distribution via immunofluorescence, quantify protein levels through Western blotting, and explore its interactions in co-immunoprecipitation assays. Such studies have revealed p60katanin's involvement in spindle formation during mitosis, axon growth in neurons, and cilia disassembly. Dysregulation of katanin is linked to neurodevelopmental disorders, microcephaly, and cancer, making these antibodies valuable for both basic research and disease mechanism exploration. Commercial p60katanin antibodies are typically raised against conserved epitopes, ensuring cross-reactivity in multiple species, including humans, mice, and zebrafish.
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