| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAT2; ECaC; ECaC1; OTRPC3; TrpV5;;TRPV5 |
| WB Predicted band size | Calculated MW: 83 kDa ; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TRPV5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于TRPV5抗体的代表性文献概览(基于既往研究整理)：
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1. **文献名称**: *"Molecular identification of the apical Ca2+ channel in 1.25-dihydroxyvitamin D3-responsive epithelia"*
**作者**: Hoenderop, J.G., et al.
**摘要**: 该研究首次利用特异性TRPV5抗体在肾脏和肠道组织中定位TRPV5通道蛋白，证实其在钙离子跨细胞主动运输中的核心作用。通过免疫组化和Western blot验证抗体特异性，发现TRPV5在远端肾小管上皮细胞顶膜高表达，且受维生素D3调控。
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2. **文献名称**: *"Immunolocalization of the epithelial Ca2+ channel (TRPV5) in the rat kidney and bone"*
**作者**: van der Eerden, B.C., et al.
**摘要**: 通过TRPV5抗体进行免疫组织化学分析，揭示了TRPV5在肾脏远端小管及骨细胞中的分布特征。研究显示，TRPV5在钙稳态调节中具有组织特异性表达模式，并探讨其与骨代谢疾病的潜在关联。
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3. **文献名称**: *"TRPV5: a Ca2+ channel for the fine-tuning of Ca2+ reabsorption"*
**作者**: Nilius, B., et al.
**摘要**: 该综述总结了TRPV5通道的生理功能及调控机制，重点提及利用TRPV5抗体进行的蛋白功能研究，包括其在肾脏钙重吸收中的关键作用及与甲状旁腺激素(PTH)的相互作用。
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4. **文献名称**: *"Calcitriol controls the epithelial calcium channel in kidney"*
**作者**: Hoenderop, J.G., et al.
**摘要**: 研究通过TRPV5抗体检测维生素D活性代谢物(1.25(OH)2D3)对肾脏TRPV5蛋白表达的影响，发现激素通过上调TRPV5表达增强钙离子吸收，为钙代谢异常疾病提供了分子机制解释。
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**备注**：以上文献为示例性质，实际引用时建议通过PubMed或Google Scholar检索最新研究，并核对抗体货号(如Santa Cruz Biotechnology或Abcam等厂商的抗体应用文献)。
The Transient Receptor Potential Vanilloid 5 (TRPV5) channel, a calcium-selective ion channel, plays a critical role in maintaining systemic calcium homeostasis by mediating calcium reabsorption in the kidneys and intestines. As a member of the TRP channel superfamily, TRPV5 is characterized by six transmembrane domains and functions as a tetrameric pore. Its activity is tightly regulated by hormones like vitamin D, parathyroid hormone, and estrogen, as well as dietary calcium levels. Dysregulation of TRPV5 has been implicated in pathological conditions such as hypercalciuria, kidney stones, and osteoporosis, making it a therapeutic target for calcium-related disorders.
TRPV5 antibodies are essential tools for studying the expression, localization, and function of this channel in both physiological and disease contexts. These antibodies are typically developed against specific epitopes, such as extracellular loops or intracellular N-/C-terminal regions, and validated for applications like Western blotting, immunohistochemistry, and immunofluorescence. High specificity is crucial to distinguish TRPV5 from its close homolog, TRPV6. which shares structural and functional similarities. Researchers use these antibodies to investigate tissue-specific distribution (e.g., renal distal tubules, duodenum) and regulatory mechanisms under varying calcium states or hormonal stimuli. In translational studies, TRPV5 antibodies aid in identifying biomarkers for calcium metabolism disorders and evaluating potential drug candidates targeting calcium transport pathways. Their utility spans molecular biology, physiology, and clinical research, underpinning advances in understanding calcium homeostasis and related diseases.
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