| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | B52; SFRS6; Splicing factor; SRP55; SRSF6;;SRSF6 |
| WB Predicted band size | Calculated MW: 40 kDa ; Observed MW: 38-45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human SRSF6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于SR蛋白重复抗体的3篇参考文献，信息基于公开学术资源整理：
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1. **文献名称**: *"Monoclonal antibodies specific for the Ser/Arg-rich domain of proteins"*
**作者**: Roth, M.B., et al.
**摘要**: 该研究报道了针对SR蛋白中Ser/Arg重复结构域(SR重复)的单克隆抗体的开发，验证了其在免疫印迹和免疫荧光中对多种SR蛋白(如SC35、ASF/SF2)的特异性识别，并应用于研究核斑点的定位。
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2. **文献名称**: *"A conserved family of antibodies targets the RS domain of SR proteins"*
**作者**: Neugebauer, K.M., et al.
**摘要**: 研究描述了一类识别SR蛋白RS结构域(富含丝氨酸/精氨酸)的保守抗体家族，通过免疫沉淀验证其与剪接复合体的相互作用，并揭示RS结构域在pre-mRNA剪接中的动态磷酸化调控。
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3. **文献名称**: *"Antibody-based profiling of SR protein phosphorylation in cellular stress responses"*
**作者**: Blencowe, B.J., et al.
**摘要**: 利用针对磷酸化SR重复结构域的抗体，分析了热休克和氧化应激下SR蛋白(如SRSF1、SRSF2)的翻译后修饰变化，发现磷酸化水平变化与剪接因子亚细胞定位及功能调控相关。
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SR protein repeat antibodies are immunological tools designed to target serine/arginine (SR)-rich domains within SR proteins, a conserved family of splicing factors critical for mRNA processing. SR proteins are characterized by one or two RNA recognition motifs (RRMs) at the N-terminus and a C-terminal domain enriched with alternating serine and arginine residues (SR repeats). These repeats mediate protein-protein interactions, facilitate recruitment to spliceosomes, and regulate splicing activity through phosphorylation.
Antibodies against SR repeats (e.g., anti-SR300. SC35) are widely used to study the localization, expression, and functional dynamics of SR proteins in constitutive and alternative splicing. They are particularly valuable in immunofluorescence, immunohistochemistry, and Western blotting to visualize nuclear speckles, where SR proteins concentrate. Such antibodies have helped elucidate SR protein roles in development, cellular stress responses, and diseases like cancer and neurodegeneration, where splicing dysregulation is implicated.
However, SR repeat antibodies may cross-react with multiple SR family members due to sequence homology in their repetitive domains. Researchers often pair them with domain-specific antibodies or genetic knockdowns to confirm target specificity. Their utility extends to investigating post-translational modifications (e.g., phosphoregulation by kinases like CLK/SRPK) that modulate splicing activity. Overall, these antibodies remain indispensable for dissecting the complex roles of SR proteins in gene expression and disease mechanisms.
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