Rabbit Monoclonal HistoneH2B(monomethylK116) Antibody

The histone H2B monomethylK116 (H2B K116me1) antibody is a specialized tool used to study a specific post-translational modification on histone H2B, a core component of nucleosomes. Histones undergo various covalent modifications, such as methylation, which regulate chromatin structure and gene expression. Monomethylation at lysine 116 (K116) of histone H2B is a less-characterized epigenetic mark compared to other histone modifications. This modification is implicated in transcriptional regulation, DNA repair, and chromatin dynamics, though its precise biological roles remain under investigation. Studies suggest H2B K116me1 may interact with other histone marks or proteins to influence nucleosome stability or serve as a docking site for effector molecules. The H2B(monomethylK116) antibody enables detection and localization of this modification via techniques like immunoblotting, immunofluorescence, or chromatin immunoprecipitation (ChIP). Its specificity for monomethylation (rather than di- or trimethylation) at K116 is critical for distinguishing unique functional states. Researchers use this antibody to explore H2B K116me1's involvement in cellular processes, developmental pathways, or diseases like cancer, where epigenetic dysregulation is common. Validation using knockout or methylation-deficient models ensures antibody reliability. Further research with this tool may uncover novel mechanisms linking H2B monomethylation to chromatin-based regulation.