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产品描述：人基质金属蛋白酶12(MMP-12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP12含量。MMP12 即基质金属蛋白酶 12，在多种生理和病理过程中发挥重要作用。研究发现它在炎症、组织重塑等机制里较为关键。它参与细胞外基质降解和重构，在肺气肿、心血管疾病等疾病发生发展中扮演角色，其作用机制是当下研究热点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，可广泛应用于基础医学研究领域，例如探究呼吸系统疾病中蛋白酶活性变化、肿瘤微环境基质重塑机制，或评估药物干预对MMP - 12表达的调控作用，为相关疾病的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EC 3.4.24.65 ELISA Kit; HME ELISA Kit; Macrophage elastase ELISA Kit; Macrophage metalloelastase ELISA Kit; Macrophage metaloelastase ELISA Kit; Matrix metallopeptidase 12 (macrophage elastase) ELISA Kit; Matrix metalloprotease 12 ELISA Kit; Matrix metalloproteinase-12 ELISA Kit; ME ELISA Kit; MGC138506 ELISA Kit; MME ELISA Kit; MMP 12 ELISA Kit; MMP-12 ELISA Kit; Mmp12 ELISA Kit; MMP12_HUMAN ELISA Kit
缩写：MMP12
Uniprot No.：P39900
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 81-96 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human MMP-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-98 | ||||
| EDTA plasma (n=4) | 97 | 92-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.6952.5942.6452.514 202.1322.0312.0821.951 101.4231.4441.4341.303 50.8130.8020.8080.677 2.50.4550.4450.4500.319 1.250.2900.3010.2960.165 0.6250.2200.2140.2170.086 00.1310.1300.131 | ||||||
货期：3-5 working days
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