| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PEN 2; PSEN2; psenen;;PEN 2 |
| WB Predicted band size | Calculated MW: 12 kDa ; Observed MW: 13 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PEN 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PEN2抗体的3篇参考文献的简要信息(注：文献标题和作者为模拟示例，实际引用需核实原始文献)：
1. **文献名称**：*"PEN2 is an essential component of the γ-secretase complex required for amyloid-β peptide production"*
**作者**：Takeo et al.
**摘要**：该研究通过基因敲除和抗体免疫沉淀技术，证实PEN2是γ-分泌酶复合物的核心组成蛋白，对淀粉样蛋白前体(APP)的切割及Aβ生成至关重要，并揭示了PEN2抗体在复合物分离检测中的关键作用。
2. **文献名称**：*"Dysregulation of PEN2 expression in Alzheimer's disease mouse models"*
**作者**：Chen et al.
**摘要**：利用PEN2特异性抗体进行免疫组化分析，发现阿尔茨海默病模型小鼠脑组织中PEN2蛋白表达水平异常升高，提示其可能通过调控γ-分泌酶活性加剧Aβ沉积和神经退行性病变。
3. **文献名称**：*"Structural and functional characterization of PEN2 interactions within the γ-secretase complex"*
**作者**：Watanabe et al.
**摘要**：通过PEN2抗体的共聚焦成像和蛋白质互作实验，解析了PEN2与其他γ-分泌酶组分(如PS1、APH-1)的结合位点，阐明其在复合物组装及底物识别中的分子机制。
如需具体文献，建议通过PubMed或Google Scholar以关键词“PEN2 antibody”、“gamma-secretase PEN2”进一步检索。
The PEN2 antibody is a crucial tool in studying the molecular mechanisms underlying Alzheimer's disease (AD) and related neurodegenerative disorders. PEN2 (Presenilin Enhancer 2) is a component of the γ-secretase complex, a multisubunit protease responsible for cleaving amyloid precursor protein (APP) to generate amyloid-β (Aβ) peptides. Accumulation of Aβ plaques in the brain is a hallmark of AD pathogenesis. PEN2 plays an essential role in stabilizing the γ-secretase complex and regulating its enzymatic activity, making it a key target for understanding Aβ production and disease progression.
Researchers utilize PEN2 antibodies primarily to detect and quantify PEN2 protein expression in cellular and tissue samples, employing techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help investigate PEN2's interaction with other γ-secretase subunits (e.g., presenilin, nicastrin, APH-1) and its involvement in substrate recognition and cleavage specificity. Studies using PEN2 knockout models or knockdown approaches, validated by such antibodies, have revealed its critical role in maintaining γ-secretase function and APP processing.
Additionally, PEN2 antibodies contribute to exploring therapeutic strategies targeting γ-secretase activity modulation. By elucidating PEN2's structure-function relationships and regulatory mechanisms, these tools aid in developing inhibitors or modulators to reduce pathogenic Aβ42 production while preserving physiologically essential γ-secretase functions. Ongoing research also examines PEN2's potential involvement beyond AD, including its roles in Notch signaling and cancer biology.
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