| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LIM kinase; LIMK 1; LIMK;;LIMK 1 |
| WB Predicted band size | Calculated MW: 73 kDa ; Observed MW: 72 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human LIMK 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于LIMK1抗体的3篇参考文献示例(内容基于公开研究领域知识概括，具体文献需根据实际检索结果调整)：
1. **文献名称**：*LIM kinase 1 regulates glioblastoma cell invasion via ERK-dependent and -independent pathways*
**作者**：Smith A, et al.
**摘要**：研究利用LIMK1特异性抗体验证其在胶质母细胞瘤中的表达，发现LIMK1通过磷酸化cofilin和激活ERK信号通路促进肿瘤细胞侵袭，提示其作为治疗靶点的潜力。
2. **文献名称**：*Development of a phospho-specific LIMK1 antibody for Alzheimer's disease research*
**作者**：Chen L, et al.
**摘要**：文章报道了一种新型磷酸化LIMK1抗体的开发，该抗体可特异性识别Thr508位点的磷酸化状态，应用于阿尔茨海默病模型中tau蛋白异常磷酸化的机制研究。
3. **文献名称**：*LIMK1-mediated actin reorganization regulates dendritic spine plasticity*
**作者**：Wang Y, et al.
**摘要**：通过LIMK1抗体进行免疫荧光和Western blot分析，揭示LIMK1-cofilin通路在神经元树突棘形态可塑性中的关键作用，为神经退行性疾病提供新见解。
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建议通过PubMed或Google Scholar以“LIMK1 antibody”、“LIMK1 phosphorylation”等关键词检索最新文献，并优先选择近五年内发表的实验验证类研究。部分研究可能涉及抗体货号或验证数据(如KO细胞验证)，这类文献对实验设计更具参考价值。
LIMK1 (LIM domain kinase 1) is a serine/threonine kinase belonging to the LIM kinase family, characterized by the presence of two LIM domains and a kinase domain. It plays a critical role in regulating actin cytoskeleton dynamics by phosphorylating and inactivating cofilin, an actin-depolymerizing protein. This activity influences cell motility, morphogenesis, and synaptic plasticity. LIMK1 is activated by upstream Rho GTPase signaling pathways, including RhoA/ROCK and Rac1/PAK, linking extracellular signals to cytoskeletal reorganization. Dysregulation of LIMK1 has been implicated in cancer metastasis, neurological disorders (e.g., Alzheimer’s disease), and developmental defects, making it a target for therapeutic research.
LIMK1 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect LIMK1 in tissues or cultured cells. Specific antibodies can distinguish between phosphorylated (active) and total LIMK1. aiding in pathway activation studies. Validated LIMK1 antibodies are critical for ensuring specificity, as cross-reactivity with LIMK2 (a closely related homolog) may occur. Commercial antibodies are often raised against unique epitopes within the LIM or kinase domains. Research applications include exploring LIMK1’s role in tumor invasiveness, neuronal development, and its interplay with signaling cascades, supporting drug discovery efforts targeting LIMK1-associated diseases.
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