| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Pitx3; PTX3;;Pituitary homeobox 3 |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Pituitary homeobox 3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PITX3抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*"Pitx3 is required for development of substantia nigra dopaminergic neurons"*
**作者**：Nunes, I., et al. (2003)
**摘要**：该研究利用PITX3特异性抗体，通过免疫组化分析发现Pitx3基因缺失导致小鼠中脑黑质多巴胺神经元发育缺陷，提示其在帕金森病病理中的潜在作用。
2. **文献名称**：*"Regulation of the dopaminergic neuron phenotype by the transcription factor Pitx3"*
**作者**：Maxwell, S.L., et al. (2005)
**摘要**：作者通过Western blot和免疫荧光技术，使用PITX3抗体证实其在维持中脑多巴胺神经元功能中的关键作用，并探讨其与帕金森病模型的关联。
3. **文献名称**：*"Pitx3 and FoxC1 in zebrafish lens development"*
**作者**：Hwang, D.Y., et al. (2009)
**摘要**：该研究在斑马鱼模型中，利用PITX3抗体进行原位杂交和免疫定位，揭示了Pitx3在晶状体发育中的时空表达模式及其与先天性白内障的联系。
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这些文献均通过PITX3抗体探究了该转录因子在不同生理和病理背景下的表达与功能，涵盖神经发育、疾病模型及眼发育等领域。
**Background of PITX3 Antibody**
PITX3 (Pituitary Homeobox 3) is a transcription factor belonging to the homeodomain-containing protein family, critical for regulating gene expression during embryonic development. It plays a pivotal role in the differentiation and maintenance of midbrain dopaminergic neurons, lens development in the eye, and the formation of the pituitary gland. Mutations or dysregulation of PITX3 are linked to congenital eye disorders (e.g., cataracts, anterior segment dysgenesis) and neurodegenerative conditions like Parkinson’s disease.
PITX3 antibodies are immunological tools designed to detect and quantify PITX3 protein expression in research applications. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study PITX3’s spatial and temporal expression patterns in tissues, particularly in the brain, lens, and developing embryos. They are essential for investigating molecular mechanisms underlying developmental biology, neurodegenerative diseases, and ocular pathologies.
Validated PITX3 antibodies are crucial for ensuring specificity and reproducibility in studies exploring PITX3’s role in cell fate determination, gene regulatory networks, and disease models. Their utility extends to screening stem cell-derived dopaminergic neurons for regenerative therapies and evaluating PITX3 as a biomarker in diagnostic research.
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