| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BAF57; SMARCE1;;SMARCE1 |
| WB Predicted band size | Calculated MW: 47 kDa ; Observed MW: 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human SMARCE1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于BAF57/SMARCE1抗体的3篇参考文献的简要信息：
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1. **文献名称**: *"The SWI/SNF chromatin-remodeling complex is required for maintenance of lineage-specific enhancers"*
**作者**: Hargreaves DC, Crabtree GR
**摘要**: 该研究通过使用BAF57抗体进行染色质免疫沉淀(ChIP)，揭示了SWI/SNF复合体在维持细胞谱系特异性增强子活性中的关键作用，证明其缺失导致基因表达失调。
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2. **文献名称**: *"SMARCE1 is required for the invasive progression of in situ cancers"*
**作者**: Inoue D, Fujino T, Sheridan MA, et al.
**摘要**: 研究利用SMARCE1抗体进行免疫组化和Western blot分析，发现SMARCE1在乳腺癌原位癌向侵袭性癌转化中不可或缺，并关联其表达与肿瘤转移风险。
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3. **文献名称**: *"BAF complexes facilitate decatenation of DNA by topoisomerase IIα"*
**作者**: Yan Z, Cui K, Murray DM, et al.
**摘要**: 通过BAF57抗体免疫共沉淀实验，研究者发现SWI/SNF复合体与拓扑异构酶IIα协同作用，调控DNA解链和基因组稳定性，揭示其在细胞分裂中的功能。
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**备注**：以上文献均涉及BAF57/SMARCE1抗体的实验应用(如Western blot、ChIP、免疫组化)，涵盖染色质重塑、癌症机制及基因组稳定性研究。具体实验细节需查阅原文。
The BAF57/SMARCE1 antibody targets a protein subunit of the SWI/SNF chromatin-remodeling complex, which plays a critical role in regulating gene expression by altering nucleosome structure. Encoded by the *SMARCE1* gene, BAF57 (also known as SMARCE1) is essential for the assembly and stability of the SWI/SNF complex, facilitating its interaction with DNA and transcription factors. This complex is involved in diverse cellular processes, including differentiation, proliferation, and DNA repair. Dysregulation of BAF57/SMARCE1 has been linked to cancers, such as breast and prostate cancer, where it modulates hormone receptor signaling (e.g., estrogen and androgen receptors), and neurological disorders.
Antibodies against BAF57/SMARCE1 are widely used in research to study its expression, localization, and function. They enable techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to assess protein levels, tissue distribution, and DNA-binding activity. Mutations in *SMARCE1* are associated with rare conditions, including Coffin-Siris syndrome and clear cell meningiomas, highlighting its role in development and disease. Researchers also utilize these antibodies to explore SWI/SNF complex integrity in cancer models or genetic screens, providing insights into epigenetic mechanisms and therapeutic targets. Its dual role in transcriptional activation and repression underscores its significance in both normal physiology and pathology.
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