| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ATP4B; Gastric H(+)/K(+) ATPase subunit beta; Potassium-transporting ATPase subunit beta; ATPase H+/K+ exchanging beta polypeptide;ATP4B |
| WB Predicted band size | Calculated MW: 33 kDa ; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human ATP4B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于ATP4B抗体的代表性文献摘要：
1. **文献名称**: *Autoantibodies to the gastric H+/K+-ATPase β-subunit in autoimmune gastritis*
**作者**: Toh BH等
**摘要**: 研究证实ATP4B抗体(靶向胃H+/K+-ATP酶β亚基)是自身免疫性胃炎(AIG)的特异性生物标志物，与胃壁细胞破坏及胃酸缺乏相关，可用于临床诊断及疾病进展监测。
2. **文献名称**: *Diagnostic value of ATP4B antibody testing in autoimmune metaplastic atrophic gastritis*
**作者**: Minalyan A等
**摘要**: 通过ELISA和免疫荧光法对比，发现ATP4B抗体对自身免疫性萎缩性胃炎具有高特异性(>90%)，可作为内镜检查前的无创筛查指标，尤其适用于早期无症状患者。
3. **文献名称**: *Molecular characterization of ATP4B autoantigen in pernicious anemia*
**作者**: Song YH等
**摘要**: 解析ATP4B抗原表位，发现其C端结构域是抗体主要结合区域。研究揭示了该抗体在恶性贫血病理机制中的作用，并开发了重组蛋白检测技术提升诊断灵敏度。
注：以上为模拟概括，实际文献需通过PubMed/Google Scholar以关键词“ATP4B antibody”、“gastric ATPase autoantibody”检索获取。建议优先选择近5年临床研究或权威综述。
The ATP4B antibody targets the beta subunit of the gastric H+/K+ ATPase, a proton pump critical for acid secretion in parietal cells of the stomach. This enzyme exchanges cytoplasmic H+ ions for luminal K+ ions, generating the highly acidic environment necessary for digestion. Autoantibodies against ATP4B are primarily associated with autoimmune gastritis, a condition where the immune system mistakenly attacks gastric parietal cells. Over time, this leads to parietal cell loss, reduced acid production (hypochlorhydria), and impaired intrinsic factor secretion, which can progress to pernicious anemia due to vitamin B12 malabsorption.
Detection of ATP4B antibodies, often via ELISA or immunofluorescence, serves as a diagnostic marker for autoimmune gastritis. These antibodies are observed in approximately 60-90% of patients with the condition and may appear years before clinical symptoms manifest. Their presence correlates with histological damage and disease progression. Notably, ATP4B antibodies frequently coexist with antibodies targeting the ATP4A subunit (alpha subunit of H+/K+ ATPase) and intrinsic factor. The autoimmune response against gastric ATPase subunits is linked to CD4+ T-cell-mediated pathology, though epitope-specific mechanisms remain under investigation. Clinically, ATP4B antibody testing aids in differentiating autoimmune gastritis from other causes of gastric atrophy and guides monitoring for hematologic and neurologic complications of B12 deficiency.
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