| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | NELFB;;NELF B |
| WB Predicted band size | 66 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human NELF B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及NELFB抗体的研究文献的简要信息，基于NELFB在转录调控中的功能整理：
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1. **文献名称**：The Negative Elongation Factor Complex regulates enhancer activity through dynamic histone methylation
**作者**：Arnold, C.D., et al.
**摘要**：研究利用NELFB抗体进行ChIP-seq分析，发现NELF复合体通过调控组蛋白H3K4me1修饰动态影响增强子活性，揭示其在基因暂停与激活转换中的作用机制。
2. **文献名称**：NELF interacts with oncogenic viral proteins to perturb host transcription elongation
**作者**：Smith, J.L., et al.
**摘要**：通过NELFB抗体免疫沉淀实验，发现EB病毒蛋白与NELF复合体结合，抑制宿主细胞转录延伸，为病毒劫持宿主基因表达提供新机制。
3. **文献名称**：Developmental regulation of the NELF complex controls neuronal differentiation
**作者**：Lee, H., et al.
**摘要**：在小鼠神经干细胞中，NELFB抗体染色显示其表达水平随分化阶段变化，敲低NELFB导致神经元分化异常，表明其在神经发育中的时序性调控作用。
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*注：以上文献信息为示例性内容，实际文献请通过PubMed或Google Scholar以"NELFB antibody"+"function"等关键词检索获取。若需具体论文，建议补充时间范围或研究领域进一步筛选。*
NELFB (Negative Elongation Factor B) is a subunit of the NELF complex (NELF-A, -B, -C/D, -E), which plays a critical role in transcriptional pausing during early elongation by RNA polymerase II (Pol II). The NELF complex, along with DSIF (DRB Sensitivity-Inducing Factor), forms a stable heterotetramer that binds promoter-proximal regions of genes, stabilizing paused Pol II and repressing transcriptional elongation. NELFB serves as a scaffold protein, mediating interactions within the NELF complex and facilitating its recruitment to chromatin. Structurally, NELFB contains a conserved N-terminal HTH (helix-turn-helix) domain involved in DNA binding and protein-protein interactions.
Antibodies targeting NELFB are essential tools for studying transcriptional regulation mechanisms, particularly in contexts like development, cellular differentiation, and stress responses. They are widely used in techniques such as chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to map NELF complex localization, assess protein expression levels, or investigate its dissociation from chromatin during pause release. Dysregulation of NELF components, including NELFB, has been implicated in cancers and developmental disorders, making these antibodies valuable for exploring disease-associated transcriptional defects. Validation often includes knockout/knockdown controls to ensure specificity due to the complex’s interdependence.
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