| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Exp5; Exportin 5; Ranbp21; xpo5;;Exportin 5 |
| WB Predicted band size | 136 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Exportin 5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Exportin5抗体的3篇文献示例(注：文献信息为模拟，供参考)：
1. **文献名称**：*Exportin5 mediates nuclear export of double-stranded RNA binding proteins and mature microRNA precursors*
**作者**：Bohnsack MT, et al.
**摘要**：该研究通过免疫共沉淀和Western blot技术，利用Exportin5特异性抗体，揭示了Exportin5在转运双链RNA结合蛋白及pre-miRNA从细胞核到细胞质中的关键作用，并验证了其与RanGTP的依赖性相互作用。
2. **文献名称**：*The role of Exportin5 in hepatitis B virus RNA export and viral replication*
**作者**：Yi J, et al.
**摘要**：作者通过RNA干扰和Exportin5抗体进行免疫荧光定位，证明Exportin5参与乙型肝炎病毒(HBV)RNA的核输出过程，抑制Exportin5可显著降低病毒复制效率，提示其作为潜在抗病毒靶点。
3. **文献名称**：*Exportin5 dysfunction links to oncogenic miRNA biogenesis in colorectal cancer*
**作者**：Okada C, et al.
**摘要**：该研究利用Exportin5抗体进行组织免疫组化分析，发现结直肠癌中Exportin5表达异常升高，导致致癌性miRNA(如miR-21)加工增强，促进肿瘤增殖和转移。
4. **文献名称**：*Structural basis for pre-miRNA recognition by Exportin5-RanGTP complex*
**作者**：Lund E, et al.
**摘要**：通过X射线晶体学结合Exportin5抗体的验证实验，解析了Exportin5-RanGTP复合物识别pre-miRNA茎环结构的分子机制，阐明其选择性运输RNA的分子基础。
(注：以上文献为示例性内容，实际引用请根据真实论文调整。)
Exportin5 (XPO5) is a member of the karyopherin-β family of nuclear transport proteins, primarily responsible for mediating the export of precursor microRNAs (pre-miRNAs) and certain viral RNAs from the nucleus to the cytoplasm. This process is critical for RNA interference pathways and post-transcriptional gene regulation. Exportin5 recognizes its cargoes via a characteristic double-stranded RNA structure, binding cooperatively with RanGTP to facilitate transport through nuclear pore complexes. Dysregulation of XPO5 has been implicated in various diseases, including cancer and viral infections, due to its role in modulating miRNA maturation and RNA shuttling.
Antibodies targeting Exportin5 are essential tools for studying its expression, localization, and functional interactions. These antibodies are typically developed in model organisms (e.g., rabbits or mice) using immunogenic peptides or recombinant protein fragments. High-quality Exportin5 antibodies exhibit specificity confirmed by techniques like Western blotting, immunoprecipitation, or immunofluorescence, often validated through knockout cell lines. Researchers employ these reagents to investigate XPO5's involvement in cellular processes, such as miRNA biogenesis defects in tumors or viral hijacking mechanisms. Commercial antibodies often include validation data across applications (e.g., IHC, flow cytometry), enabling diverse experimental approaches. Proper antibody selection depends on target epitopes, species cross-reactivity, and intended methodologies, with controls crucial for interpreting results in complex biological systems.
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