| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PKC alpha; PKC beta; PKC delta; PKC epsilon; PKC gamma; PKC zeta; PKC2; PKCA; PKCB; PKCD; PKCE; PKCG; PRKCA; PRKCB; PRKCD; PRKCE; PRKCG; PRKCZ; ;PKC zeta |
| WB Predicted band size | Calculated MW: 68 kDa ; Observed MW: 77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PKC zeta |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4条关于PKC(Protein Kinase C)抗体的参考文献及其简要摘要：
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1. **文献名称**：**"Protein kinase C: structure, function, and regulation"**
**作者**：Nishizuka, Y.
**摘要**：该综述总结了PKC家族的结构、功能及其调控机制，重点讨论了不同PKC亚型(如PKCα、β、γ)的激活途径和生理作用，并提及了特异性抗体在亚型鉴定和定位研究中的应用。
2. **文献名称**：**"Subtypes of protein kinase C and their roles in transmembrane signalling"**
**作者**：Newton, A.C.
**摘要**：文章探讨了PKC亚型在细胞信号转导中的特异性作用，通过使用亚型选择性抗体(如抗PKCδ、PKCε抗体)，揭示了不同亚型在细胞分化、凋亡及肿瘤发生中的独特功能。
3. **文献名称**：**"The extended protein kinase C superfamily"**
**作者**：Mellor, H. and Parker, P.J.
**摘要**：该研究利用多种PKC抗体(如针对催化结构域或调节结构域的抗体)，系统分析了PKC超家族成员的结构差异及其在磷酸化底物识别中的作用，强调了抗体在检测PKC活性状态中的重要性。
4. **文献名称**：**"Intracellular receptor for activated protein kinase C: targeting protein kinase C signaling to specific subcellular loci"**
**作者**：Mochly-Rosen, D. et al.
**摘要**：通过开发针对PKC锚定蛋白(RACKs)的抗体，研究揭示了PKC亚型在亚细胞定位中的调控机制，为理解PKC信号的空间特异性提供了实验依据。
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以上文献均涉及PKC抗体的应用，涵盖亚型鉴定、活性检测及亚细胞定位等领域。具体引用时建议核对原文以确保准确性。
Protein kinase C (PKC) antibodies are essential tools in biomedical research, targeting a family of serine/threonine kinases critical for regulating cellular signaling pathways. PKC isoforms (e.g., α, β, γ, δ) are involved in diverse processes, including cell proliferation, differentiation, apoptosis, and immune responses. These enzymes are activated by lipid-derived secondary messengers like diacylglycerol (DAG) and calcium ions, modulating downstream targets by phosphorylation. Dysregulation of PKC activity is linked to cancer, neurological disorders, and metabolic diseases, driving interest in their study.
PKC antibodies are designed to detect specific isoforms or pan-PKC epitopes, enabling applications such as Western blotting, immunohistochemistry, and flow cytometry. Their development relies on immunizing hosts with peptide antigens derived from conserved or variable regions of PKC isoforms. Monoclonal antibodies offer high specificity, while polyclonal versions detect broader epitopes. Challenges include cross-reactivity due to isoform homology, necessitating rigorous validation.
These antibodies have advanced understanding of PKC's role in disease mechanisms, drug resistance, and therapeutic targeting. For instance, PKCα is implicated in tumor invasiveness, while PKCδ relates to oxidative stress responses. Researchers also use inhibitory antibodies to explore PKC signaling modulation. Overall, PKC antibodies remain pivotal in elucidating kinase biology and developing precision therapies.
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