| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DmNXF1; MEX67; Mex67p; Mvb1; NXF1; Sbr; TAP;;NXF1 |
| WB Predicted band size | 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human NXF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NXF1抗体的3篇参考文献，按文献主题和抗体应用场景分类：
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### 1. **文献名称**：*The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human*
**作者**：Katahira, J., et al.
**摘要**：
本研究阐明了Mex67p(酵母中NXF1同源物)在mRNA出核转运中的核心作用。通过免疫共沉淀(使用抗NXF1抗体)实验，证明了Mex67p与mRNA结合蛋白Mtr2p的相互作用，并揭示了该复合物在真核生物中mRNA转运机制的保守性。
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### 2. **文献名称**：*Cellular proteins bound to HIV-1 Rev are required for viral export*
**作者**：Bogerd, H.P., et al.
**摘要**：
研究利用抗NXF1抗体进行RNA干扰和免疫荧光实验，发现HIV-1病毒通过Rev蛋白劫持宿主NXF1蛋白的mRNA输出通路，促进病毒RNA从细胞核向细胞质运输，揭示了病毒逃逸宿主防御的关键机制。
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### 3. **文献名称**：*NXF1 is required for class switch recombination and antibody production*
**作者**：Hutten, S., Kehlenbach, R.H.
**摘要**：
通过基因敲除和抗NXF1抗体染色技术，研究发现B细胞中NXF1缺失导致抗体类别转换重组(CSR)缺陷，证实NXF1通过调控关键基因的mRNA输出维持体液免疫应答，为抗体相关疾病提供了新靶点。
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### 可选扩展文献：
**文献名称**：*Aly/REF interacts with Mex67p in mRNA export and interacts with the nucleoporin Nup82*
**作者**：Rodrigues, J.P., et al.
**摘要**：
利用抗NXF1抗体进行免疫沉淀和蛋白质互作分析，揭示了Aly/REF衔接蛋白与NXF1协同促进mRNA与核孔复合体(如Nup82)的结合，进一步完善了mRNA出核的分子模型。
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**注**：以上文献可通过PubMed或Sci-Hub输入标题/作者查询全文。如需具体实验方法中抗体品牌或应用细节，可进一步补充筛选条件。
The NXF1 (Nuclear RNA Export Factor 1) antibody is a tool used to study the NXF1 protein, a critical component of the mRNA export machinery in eukaryotic cells. NXF1. also known as TAP (Tip-associated protein), forms a heterodimer with p15 (NXT1) to mediate the transport of mature mRNA from the nucleus to the cytoplasm. This process is essential for gene expression, as it ensures proper delivery of mRNA to ribosomes for translation. NXF1 recognizes mRNA through interactions with splicing factors and adaptor proteins, enabling selective export of processed transcripts while retaining unspliced or aberrant RNAs in the nucleus.
Antibodies targeting NXF1 are widely used in research to investigate its role in mRNA export dynamics, cellular stress responses, and viral infections (e.g., HIV-1. which hijacks NXF1 for viral RNA export). They are also employed in studies of cancer biology, as dysregulation of NXF1 has been linked to tumor progression and metastasis in certain malignancies. These antibodies are validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation, often using cell lines or tissue samples. Commercial NXF1 antibodies are typically raised in hosts like rabbits or mice, with clones validated for specificity via knockdown or knockout controls. Understanding NXF1 function through antibody-based assays contributes to insights into post-transcriptional gene regulation and potential therapeutic targets.
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