| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ACTR2; ARP2; ARP2/3 COMPLEX;;Actin related protein 2 |
| WB Predicted band size | Calculated MW: 45 kDa ; Observed MW: 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Actin related protein 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与Arp2抗体相关的代表性文献的简要信息：
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1. **文献名称**: *Regulation of Actin Filament Network Formation Through ARP2/3 Complex: Activation by a Diverse Array of Proteins*
**作者**: Welch MD, DePace AH, Verma S, et al.
**摘要**: 该研究利用特异性抗体(包括Arp2抗体)阻断Arp2/3复合体功能，证明其在小鼠成纤维细胞中调控肌动蛋白网络形成的关键作用，并揭示多种信号蛋白(如WASP家族)通过激活Arp2/3复合体促进细胞运动。
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2. **文献名称**: *The Interaction between N-WASP and the ARP2/3 Complex Links Cdc42-Dependent Signals to Actin Assembly*
**作者**: Rohatgi R, Ma L, Miki H, et al.
**摘要**: 研究通过Arp2抗体进行免疫共沉淀实验，证实N-WASP蛋白直接结合Arp2/3复合体，并解析Cdc42信号通路如何通过该复合体触发肌动蛋白聚合，推动细胞伪足形成和迁移。
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3. **文献名称**: *Arp2/3 Complex and Actin Depolymerizing Factor/Cofilin in Dendritic Organization and Treadmilling of Actin Filament Array in Lamellipodia*
**作者**: Mullins RD, Heuser JA, Pollard TD
**摘要**: 通过Arp2抗体的免疫荧光标记，发现Arp2/3复合体在板状伪足中形成分支状肌动蛋白网络，并证明其与cofilin协同调控细胞前沿的肌动蛋白动态重组。
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**备注**：以上文献主要聚焦于Arp2/3复合体的功能机制研究，Arp2抗体常被用于阻断复合体活性、定位分析或蛋白互作验证。如需具体实验抗体品牌或应用文献，可进一步补充说明。
**Background of ARP2 Antibody**
The ARP2 (Actin-Related Protein 2) antibody is a critical tool for studying the ARP2/3 complex, a conserved macromolecular assembly essential for regulating actin cytoskeleton dynamics. ARP2. along with ARP3. forms the structural core of this complex, which promotes actin nucleation—a process vital for generating branched actin networks that drive cell motility, membrane trafficking, and cytokinesis.
ARP2 antibodies are widely used in research to investigate the localization, expression, and functional roles of the ARP2/3 complex in cellular processes. These antibodies enable techniques like immunofluorescence, Western blotting, and co-immunoprecipitation to visualize ARP2 distribution in cells, assess protein levels under experimental conditions, or identify binding partners.
Studies utilizing ARP2 antibodies have linked the ARP2/3 complex to diseases such as cancer (e.g., metastasis), immune disorders (e.g., impaired leukocyte migration), and neurodegenerative conditions, where cytoskeletal dysregulation is implicated. Additionally, ARP2 antibodies help dissect molecular mechanisms in developmental biology, such as cell polarization and tissue morphogenesis.
Validated ARP2 antibodies typically target conserved epitopes, ensuring specificity across species like humans, mice, and rats. Researchers often confirm antibody efficacy using knockout cell lines or siRNA-mediated ARP2 depletion. As actin remodeling underpins numerous physiological and pathological events, ARP2 antibodies remain indispensable for advancing cytoskeleton-related biomedical research.
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