| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DIAPH1; DFNA1; DIA1; DIAP1; DIAPH1; DRF1; hDIA1; LFHL1;;Diaphanous homolog 1 |
| WB Predicted band size | Calculated MW: 141 kDa ; Observed MW: 155 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Diaphanous homolog 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DIAPH1抗体的3篇参考文献示例(基于公开研究主题，具体作者和标题为示例性概括)：
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1. **文献名称**：*"DIAPH1 regulates actin polymerization in cellular migration and hearing function"*
**作者**：Smith J, et al.
**摘要**：本研究利用DIAPH1特异性抗体进行Western blot和免疫荧光分析，发现DIAPH1通过调控肌动蛋白动力学影响细胞迁移，并在小鼠内耳毛细胞中定位，提示其与听力功能相关。
2. **文献名称**：*"A novel DIAPH1 mutation associated with autosomal dominant hearing loss"*
**作者**：Lee S, et al.
**摘要**：通过基因测序和抗体免疫组化技术，作者发现DIAPH1基因突变导致蛋白质异常表达，破坏耳蜗细胞骨架结构，从而引发遗传性听力损失。
3. **文献名称**：*"DIAPH1-mediated actin dynamics promote cancer metastasis"*
**作者**：Garcia R, et al.
**摘要**：使用DIAPH1抗体进行肿瘤组织芯片分析，发现高表达DIAPH1与乳腺癌转移相关，其机制可能通过增强细胞侵袭性伪足形成。
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**注**：以上文献为示例性内容，实际引用时需以具体检索到的权威文献为准(建议通过PubMed或Google Scholar以关键词“DIAPH1 antibody”或“DIAPH1 function”查找最新研究)。
The DIAPH1 antibody is a research tool targeting the Diaphanous homolog 1 (DIAPH1) protein, a member of the formin family involved in cytoskeletal dynamics. DIAPH1 regulates actin polymerization, microtubule stabilization, and cell motility by interacting with Rho GTPases. It plays critical roles in cellular processes such as cytokinesis, cell migration, and signal transduction. Dysregulation of DIAPH1 has been linked to various pathologies, including cancer metastasis, cardiovascular disorders, and neurodevelopmental conditions like hearing loss (e.g., autosomal dominant deafness-1).
DIAPH1 antibodies are widely used in biomedical research to investigate the protein's expression, localization, and function. They enable detection via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specific applications include studying DIAPH1's role in tumor invasiveness, endothelial cell permeability, or platelet activation. Some antibodies distinguish between phosphorylated (active) and inactive forms, aiding mechanistic studies. Commercially available DIAPH1 antibodies vary in clonality (monoclonal/polyclonal) and host species, requiring validation using knockout controls or siRNA-treated samples. Recent studies also explore DIAPH1 as a potential therapeutic target, particularly in inflammation and fibrosis, driving demand for reliable antibody reagents. Proper validation remains essential due to occasional cross-reactivity with other formin proteins.
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