| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CRABP; Crabp1; CRABPI; RBP5;;CRABP1 |
| WB Predicted band size | Calculated MW: 16 kDa ; Observed MW: 14 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human CRABP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于CRABP1抗体的参考文献示例(内容为假设性示例，仅供参考)：
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1. **"CRABP1 regulates retinoic acid signaling in neuronal differentiation"**
*作者：Kyle, J. et al. (2018)*
**摘要**：通过Western blot和免疫荧光技术，使用CRABP1抗体证明其在神经元分化过程中调控视黄酸信号通路，影响小鼠胚胎干细胞的分化效率。
2. **"CRABP1 expression correlates with prognosis in colorectal cancer"**
*作者：Zhang, Y. et al. (2020)*
**摘要**：利用CRABP1抗体进行免疫组化分析，发现CRABP1在结直肠癌组织中低表达，且与患者生存率正相关，提示其可能作为预后标志物。
3. **"CRABP1 knockout mice exhibit impaired epidermal barrier function"**
*作者：Miller, R. et al. (2015)*
**摘要**：通过CRABP1抗体检测蛋白表达缺失，揭示CRABP1基因敲除小鼠皮肤屏障功能缺陷，表明其在表皮细胞视黄酸代谢中的关键作用。
4. **"CRABP1 and FABP5 cooperatively modulate cellular retinoic acid bioavailability"**
*作者：Saito, M. et al. (2017)*
**摘要**：结合CRABP1抗体和质谱技术，研究发现CRABP1与FABP5协同调控细胞内视黄酸的分布，影响靶基因转录活性。
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注：上述文献为示例，实际引用时请核实真实来源及内容。
Cellular retinoic acid-binding protein 1 (CRABP1) is a cytosolic protein belonging to the fatty acid-binding protein family, primarily involved in binding and transporting retinoic acid (RA), a bioactive metabolite of vitamin A. RA plays critical roles in cell differentiation, proliferation, and embryonic development by regulating gene expression through nuclear receptors like RARs (retinoic acid receptors). CRABP1 modulates RA bioavailability by sequestering it in the cytoplasm, thereby influencing its nuclear signaling or metabolic degradation. Unlike its homolog CRABP2. which facilitates RA delivery to RARs, CRABP1 is often associated with RA catabolism via cytochrome P450 enzymes.
CRABP1 antibodies are essential tools for studying its expression, localization, and function in developmental biology, oncology, and neurodegenerative diseases. These antibodies are used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect CRABP1 in tissues and cell lines. Dysregulation of CRABP1 has been implicated in cancers (e.g., breast, lung) and neurodevelopmental disorders, making its detection crucial for understanding disease mechanisms. Validated antibodies typically target specific epitopes, ensuring specificity through knockout controls or siRNA validation. Commercial CRABP1 antibodies are available in monoclonal or polyclonal formats, often raised in rabbits or mice. Research using these antibodies has expanded insights into CRABP1's role in RA signaling homeostasis and its potential as a therapeutic target or biomarker.
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