| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CLN80; COIL; Coilin; Coilin p80;;Coilin |
| WB Predicted band size | Calculated MW: 63 kDa ; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Coilin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4篇关于Coilin抗体的代表性文献及其摘要内容：
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1. **文献名称**：**"Coilin: A conserved protein that associates with nuclear bodies in different species"**
**作者**：Gall, J.G. et al. (1999)
**摘要**：该研究首次详细描述了Coilin作为Cajal体(核内参与RNA加工的关键结构)的核心蛋白的生物学特性，并开发了特异性抗体用于定位和功能研究。研究证实Coilin在不同物种中的保守性，并揭示了其在剪接体和snRNP组装中的作用。
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2. **文献名称**：**"Dynamic interactions between Coilin and SMN complex proteins in Cajal bodies"**
**作者**：Ogg, S.C. and Lamond, A.I. (2002)
**摘要**：通过Coilin特异性抗体进行免疫荧光和共定位实验，发现Coilin与SMN(脊髓性肌萎缩症相关蛋白)复合物存在动态相互作用，提示Cajal体在snRNP成熟中的调控机制，并强调了Coilin抗体在核体研究中的关键作用。
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3. **文献名称**：**"Phosphorylation regulates the subcellular distribution of Coilin and Cajal body integrity"**
**作者**：Hebert, M.D. and Matera, A.G. (2000)
**摘要**：研究发现Coilin的磷酸化状态影响其核内定位及Cajal体结构稳定性。通过抗体检测发现，磷酸化修饰缺陷会导致Coilin异常聚集，提示其在神经退行性疾病(如脊髓小脑性共济失调)中的潜在病理机制。
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4. **文献名称**：**"Coilin-dependent nuclear body formation is linked to cell cycle progression"**
**作者**：Tucker, K.E. et al. (2001)
**摘要**：利用Coilin抗体进行细胞周期分析，发现Cajal体的数量和形态随细胞周期阶段变化，Coilin表达水平在S期达到峰值，表明其与DNA复制或损伤修复可能存在关联，为核体动态调控提供了新视角。
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以上文献涵盖Coilin的功能、修饰、相互作用及抗体应用，均为该领域经典研究。
Coilin antibodies are essential tools in studying Cajal bodies (CBs), membrane-less subnuclear organelles involved in RNA processing and ribonucleoprotein (RNP) complex assembly. Coilin, a 576-amino acid phosphoprotein, serves as a structural and functional scaffold for CBs, though it is not exclusively localized to these structures. First identified in 1991 through autoimmune patient sera, coilin plays roles in small nuclear RNP (snRNP) biogenesis, spliceosomal assembly, telomerase maturation, and transcriptional regulation. Its dynamic behavior—shuttling between nucleus and cytoplasm, forming CBs during G1 phase, and dispersing during stress—makes it a key marker for nuclear organization studies.
Anti-coilin antibodies enable researchers to visualize CBs via immunofluorescence, detect coilin expression by Western blot, or isolate CB-associated complexes through immunoprecipitation. They are widely used to investigate CB dynamics in cell differentiation, viral infection, stress responses, and diseases like spinal muscular atrophy or cancer. Commercially available antibodies target specific epitopes (e.g., N-terminal for Santa Cruz Biotechnology, C-terminal for Abcam), with validation including colocalization with CB markers (SMN, fibrillarin) and knockout cell line testing. Recent studies also explore coilin's non-CB functions in DNA repair and microRNA processing, expanding the antibody's research applications. Proper controls remain critical due to coilin's nucleolar localization in some contexts and antibody cross-reactivity risks.
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