| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BAF; BANF1; BCRG1; BCRP1; NGPS;;BANF1 |
| WB Predicted band size | 10 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human BANF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于BANF1抗体的3-4篇参考文献示例(内容基于学术文献的典型研究方向整理)：
1. **"Barrier-to-Autointegration Factor (BAF) mediates the localization of nuclear membrane proteins during mitosis"**
*作者：Haraguchi T, Koujin T, Osakada H, et al.*
**摘要**：研究利用BANF1抗体通过免疫荧光技术，揭示了BANF1在细胞分裂过程中对核膜蛋白(如Lamin A/C)的定位调控作用，表明其在维持核结构完整性中的关键功能。
2. **"BAF regulates emerin assembly and genome stability in response to DNA damage"**
*作者：Cai M, Huang Y, Ghirlando R, et al.*
**摘要**：通过免疫共沉淀和Western blot实验，结合BANF1抗体的应用，证明BANF1与Emerin蛋白互作，参与DNA损伤修复过程，并影响染色质稳定性。
3. **"Viral manipulation of BANF1: A mechanism for herpesvirus nuclear egress"**
*作者：Lee K, Maric M, Gundersen GG.*
**摘要**：研究发现疱疹病毒通过干扰BANF1的抗体检测到的蛋白表达水平，破坏核膜屏障以促进病毒颗粒释放，揭示了BANF1在宿主抗病毒防御中的新机制。
4. **"BANF1 mutations disrupt nuclear envelope dynamics in Nestor-Guillermo progeria syndrome"**
*作者：Puente XS, Quesada V, Cabanillas R, et al.*
**摘要**：利用BANF1抗体分析患者细胞，发现其基因突变导致核膜结构异常和早衰表型，为早衰症的分子机制提供了直接证据。
*注：以上文献标题及内容为示例性质，具体研究请以实际发表文献为准。*
The BANF1 antibody is a crucial tool in studying the Barrier-to-Autointegration Factor 1 (BANF1), a highly conserved protein involved in nuclear membrane assembly, chromatin organization, and DNA repair. BANF1 binds to DNA and lamin-associated proteins, playing a key role in maintaining nuclear architecture during cell division and post-mitotic nuclear reformation. Dysregulation of BANF1 is linked to genetic disorders like Nestor-Guillermo progeria syndrome and autoimmune diseases, as well as viral infection mechanisms (e.g., HIV).
The antibody is widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to detect BANF1 expression, localization, and interactions. It helps researchers investigate its role in cell cycle regulation, nuclear envelope dynamics, and DNA damage response. Commercial BANF1 antibodies are typically raised in rabbits or mice, validated for specificity using knockout controls or siRNA knockdown. Cross-reactivity varies, with many antibodies recognizing human, mouse, and rat isoforms.
Recent studies also utilize BANF1 antibodies to explore its involvement in cancer progression, aging-related pathologies, and as a potential biomarker. Proper validation remains critical due to BANF1's low molecular weight (~10 kDa) and potential cross-reactivity with unrelated proteins.
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