| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | OST1; RBPH1; Ribophorin I; Rpn1;;RPN1 |
| WB Predicted band size | 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthesized peptide derived from human RPN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Ribophorin I抗体的参考文献概览(虚构示例，供格式参考)：
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1. **文献名称**: *Ribophorin I as a Novel Biomarker in Hepatocellular Carcinoma*
**作者**: Li, X. et al.
**摘要**: 研究利用Ribophorin I特异性抗体，通过免疫组化发现其在肝癌组织中高表达，并证实其与患者预后不良相关，提示其作为潜在诊断标志物的价值。
2. **文献名称**: *Role of Ribophorin I in ER-associated Protein Glycosylation*
**作者**: Müller, T. & Zimmermann, R.
**摘要**: 通过Western blot和免疫荧光技术，使用Ribophorin I抗体探究其在内质网中与OST复合物相互作用，揭示其在N-糖基化中的调控机制。
3. **文献名称**: *Antibody-based Screening of Ribophorin I in Multiple Myeloma*
**作者**: García-Sanz, A. et al.
**摘要**: 开发高特异性Ribophorin I单克隆抗体，用于多发性骨髓瘤患者样本检测，发现其表达水平与肿瘤侵袭性正相关，为靶向治疗提供依据。
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注：以上文献为示例，实际引用需查询真实数据库(如PubMed)。建议使用关键词“Ribophorin I antibody”结合研究领域筛选文献。
Ribophorin I is a conserved transmembrane glycoprotein predominantly located in the rough endoplasmic reticulum (ER). It forms a heterodimeric complex with Ribophorin II, which is essential for the assembly and function of the oligosaccharyltransferase (OST) complex—a critical enzyme machinery mediating N-linked glycosylation. This post-translational modification involves the transfer of preassembled oligosaccharides to specific asparagine residues on nascent polypeptides, playing a vital role in protein folding, quality control, and cellular trafficking. Ribophorin I also interacts with ribosomes and Sec61 translocons, facilitating the coupling of protein translocation and glycosylation.
Antibodies targeting Ribophorin I are valuable tools for studying ER-associated processes, including glycoprotein biosynthesis, ER stress responses, and diseases linked to glycosylation defects. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess protein expression, localization, and molecular interactions. In cancer research, Ribophorin I antibodies have been employed to investigate its overexpression in certain malignancies, such as multiple myeloma, where it may serve as a potential biomarker. Additionally, these antibodies aid in exploring Ribophorin I's role in ER homeostasis and its implications in neurodegenerative disorders. Their specificity and reliability make them indispensable for elucidating the molecular mechanisms underlying protein maturation and ER-related pathologies.
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