| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PC; PCB; Pcx; Pyruvate carboxylase;;Pyruvic carboxylase |
| WB Predicted band size | 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Pyruvic carboxylase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4条关于PCB(多氯联苯)抗体的参考文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**：*Development of a Monoclonal Antibody-Based ELISA for the Analysis of Polychlorinated Biphenyls*
**作者**：Stanker, L.H.; Watkins, B.E.
**摘要**：研究报道了一种针对PCB的单克隆抗体的开发，并通过ELISA技术实现了对环境中PCB的高灵敏度检测，检测限低于10 ppb，适用于水体和土壤样本的快速筛查。
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2. **文献名称**：*Immunoassay for Polychlorinated Biphenyls in Soil Samples*
**作者**：Van Emon, J.M.; López-Avilés, A.
**摘要**：提出一种基于多克隆抗体的免疫分析方法，用于土壤中PCB的定量检测。该方法与气相色谱法结果高度一致，证明其在环境监测中的可靠性和实用性。
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3. **文献名称**：*PCB Immunosensors: Rapid Detection Using Antibody-Coated Electrodes*
**作者**：Rogers, K.R.; Gerlach, C.L.
**摘要**：探讨了基于抗体的电化学免疫传感器技术，通过固定化PCB抗体实现实时检测，灵敏度达ng/mL级别，适用于工业废水中的PCB污染监测。
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4. **文献名称**：*Cross-Reactivity of Anti-PCB Antibodies with Structurally Similar Compounds*
**作者**：Krämer, P.M.; Franek, M.
**摘要**：分析了PCB抗体的交叉反应特性，发现其对部分二噁英和联苯类化合物存在交叉反应，提示在免疫检测中需结合色谱技术以提高特异性。
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以上文献涵盖了PCB抗体的开发、检测技术(ELISA、免疫传感器)及交叉反应研究，可支持环境检测或生物分析领域的研究参考。
**Background of Phospho-Specific Antibodies (pAbs)**
Phospho-specific antibodies, often referred to as phospho-antibodies, are critical tools in molecular biology and biomedical research for detecting post-translational modifications, specifically phosphorylation. Phosphorylation, mediated by kinases and phosphatases, regulates protein activity, localization, and interactions, playing a pivotal role in cellular signaling, cell cycle progression, and disease mechanisms.
Developed in the late 20th century, these antibodies are designed to recognize phosphorylated amino acid residues (e.g., serine, threonine, tyrosine) within target proteins. Their specificity enables researchers to study dynamic phosphorylation events in pathways like MAPK/ERK, PI3K/AKT, and immune signaling. Applications include Western blotting, immunofluorescence, and flow cytometry, aiding in understanding diseases such as cancer, neurodegeneration, and metabolic disorders.
Challenges in developing phospho-antibodies include ensuring epitope specificity due to transient, low-abundance phosphorylation. Advances in peptide synthesis and hybridoma technology have improved their reliability. Today, phospho-antibodies are indispensable in drug discovery, biomarker validation, and mechanistic studies, offering insights into cellular responses to stimuli, stress, or therapeutic agents. Their use continues to expand with the growing emphasis on precision medicine and signaling network analysis.
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