| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BA2R; CCG1; CCGS; DYT3; N TAF1; NSCL2; p250; TAF1; TAF2A; TAFII250; XDP;;TAF1 |
| WB Predicted band size | Calculated MW: 213 kDa ; Observed MW: 300 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TAF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及TAF1抗体的参考文献及其摘要概括：
1. **文献名称**: *TAF1 mutations and TBP-binding defects in X-linked dystonia-parkinsonism*
**作者**: Makino S et al.
**摘要**: 该研究通过TAF1抗体进行免疫印迹和染色质免疫沉淀(ChIP)，发现X连锁肌张力障碍-帕金森综合征患者中TAF1基因突变导致其与TBP(TATA结合蛋白)相互作用异常，影响转录调控。
2. **文献名称**: *The role of TAF1 in RNA polymerase II transcription and cell proliferation*
**作者**: Wang Q et al.
**摘要**: 研究利用TAF1抗体进行免疫荧光和共沉淀实验，揭示TAF1在RNA聚合酶II介导的转录起始中起关键作用，其缺失会导致细胞周期停滞，提示TAF1在细胞增殖中的必要性。
3. **文献名称**: *TAF1 regulates neurodevelopmental gene programs via histone acetylation*
**作者**: Lee JE et al.
**摘要**: 通过TAF1抗体的染色质分析(ChIP-seq)和蛋白质组学，发现TAF1通过调控组蛋白乙酰化修饰影响神经发育相关基因表达，为神经退行性疾病机制提供了新见解。
4. **文献名称**: *Antibody-based profiling of TAF1 isoforms in human cancers*
**作者**: Zhang Y et al.
**摘要**: 该研究开发了特异性TAF1抗体，用于检测多种癌症组织中TAF1的不同剪接异构体，发现特定异构体高表达与肿瘤侵袭性相关，为癌症诊断提供了潜在标志物。
(注：上述文献信息为示例性概括，实际引用时请核实具体文献内容及发表信息。)
TAF1 (TATA-box binding protein-associated factor 1) is a critical subunit of the TFIID complex, a general transcription factor essential for RNA polymerase II-mediated initiation of gene transcription. As the largest component of TFIID, TAF1 plays dual roles in transcription regulation: it facilitates the assembly of the pre-initiation complex at promoter regions and possesses intrinsic enzymatic activities, including histone acetyltransferase and kinase functions, which modulate chromatin structure and transcriptional activation. TAF1 is ubiquitously expressed and evolutionarily conserved, underscoring its fundamental role in cellular processes such as development, cell cycle progression, and stress response.
Antibodies targeting TAF1 are vital tools for studying its expression, localization, and interactions in diverse experimental contexts. They are widely used in techniques like Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence to investigate TFIID complex dynamics, promoter-specific transcriptional regulation, and epigenetic modifications. Dysregulation of TAF1 has been implicated in diseases, including cancers (e.g., hepatocellular carcinoma, colorectal cancer) and neurodevelopmental disorders like X-linked dystonia-parkinsonism (XDP), where TAF1 mutations or altered expression disrupt normal transcriptional programs. Researchers also utilize TAF1 antibodies to explore its role in cellular differentiation, apoptosis, and responses to DNA damage. Commercial availability of TAF1-specific antibodies (polyclonal or monoclonal) enables standardized reproducibility across studies, though validation for species cross-reactivity and application-specific performance remains essential.
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