| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHX 10; HOX10; MCOP2; MCOPCB3; RET1; Vsx2;;VSX2 |
| WB Predicted band size | Calculated MW: 39 kDa ; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human VSX2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3条关于CHX10抗体的参考文献及其摘要概括：
1. **"Vsx2 (Chx10) requires Notch signaling to prevent gliogenesis in the retina"**
*作者：Riesenberg AN, Liu Z, Kopan R, Brown NL*
摘要：研究利用Chx10抗体追踪视网膜发育中的神经前体细胞，发现Notch信号通路通过调控Chx10表达抑制胶质细胞生成，维持视网膜神经细胞分化潜能。
2. **"The role of the homeobox gene Chx10 in the development of the murine retina"**
*作者：Burmeister M, Novak J, Liang MY, Basu S, Ploder L, Hawes NL*
摘要：通过Chx10抗体免疫组化分析，揭示Chx10在小鼠视网膜双极细胞分化中的关键作用，并发现Chx10突变导致视网膜分层异常和光感受器退化。
3. **"Antibody markers of retinal cell types"**
*作者：Hendrickson A, Yan YH, Erickson A*
摘要：系统评估了包括Chx10抗体在内的多种视网膜标记抗体，验证了Chx10抗体在灵长类和小鼠视网膜中对双极细胞的特异性标记能力，并比较了不同物种间的表达差异。
(注：以上文献信息为示例性概括，实际引用需核对具体文献来源。)
The CHX10 antibody is a crucial tool in neuroscience and developmental biology research, targeting the CHX10 protein (also known as VSX2), a homeodomain transcription factor encoded by the *VSX2* gene. CHX10 plays a pivotal role in retinal development, particularly in the differentiation and maintenance of bipolar cells within the retina, as well as in spinal cord interneuron specification. Its expression is tightly regulated during embryogenesis, and mutations in *VSX2* are linked to ocular disorders such as microphthalmia (abnormally small eyes) and congenital blindness.
The CHX10 antibody is widely used to identify and study CHX10-expressing cells in tissue sections, aiding investigations into retinal circuitry, developmental defects, and regenerative therapies. Researchers employ it in techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to visualize protein localization and expression levels. It has also become valuable in stem cell research, where tracking CHX10 helps assess the differentiation of retinal progenitor cells in vitro.
Beyond basic science, this antibody supports disease modeling and drug discovery for vision-related pathologies. Its specificity and reliability make it a cornerstone in understanding how CHX10 contributes to visual system development and dysfunction, offering insights into potential therapeutic strategies for retinal degenerative diseases.
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